In the present study, effect of doxorubicin alone and in combination with vitamin E was assessed on cardiac and liver biomarkers. Rats were divided into four groups of 8 male rats in each. Group 1 served as control, group 2 (doxorubicin toxic control) was given doxorubicin dissolved in sterile distilled water @ 2 mg/kg b.wt intraperitoneally on day 1, 7, 14, 21. Groups 3 and 4 were administered doxorubicin as in group 2 + vitamin E @ 150 and 500 mg/kg b.wt., respectively through oral route daily for 28 days. The concentration of thiobarbituric acid reacting substances (TBARS), protein carbonyls and troponins (serum salt soluble proteins), and the activity of serum lactate dehydrogenase (LDH), serum aspartate transaminase (AST) were increased in group 2 as compared to control group. The concentration of reduced glutathione (GSH), and the activity of CYP450, Na+/K+ ATPase and Mg+2 ATPase were reduced in group 2 as compared to control group. The groups 3 and 4 revealed improvement in the parameters in study. In conclusion, the study revealed that doxorubicin induces cardiac and hepatic damage by inducing oxidative stress and supplementation of vitamin E is beneficial in countering the adverse effects.
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