Orbifloxacin is a fluoroquinolone drug, exclusively developed for the veterinary medicine. But since the discovery of the drug, there are very few research is available on the pharmacokinetics of orbifloxacin and there is lack of data availability of on retention time. Therefore, we have conducted the study to elucidate retention time of orbifloxacin in healthy mehsana goats and patanwadi sheep. The study was conducted on 6 healthy goats and 6 healthy sheep. Blood samples (5 mL) were collected by using heparinized tubes from jugular vein without drug administration. Different standards of orbifloxacin were prepared by using pure form drug. Orbifloxacin standard concentrations in plasma were determined by high performance liquid chromatography with UV detector. In goats, the retention time was 2.426, 2.431, 2.432, 2.432, 2.432 and 2.431 min at different orbifloxacin standard concentrations 0.0625, 0.125, 2.5, 5.0, 10.0 and 20.0 µg/mL, respectively. In sheep, the retention time was 2.412, 2.411, 2.412, 2.415, 2.415 and 2.415 min at different orbifloxacin standard concentrations 0.0625, 0.125, 2.5, 5.0, 10.0 and 20.0 µg/mL, respectively. The mean retention time of orbifloxacin standards was 2.43 ± 0.0023 min and 2.41 ± 0.0019 min in goats and sheep, respectively. The study results indicate that retention time was comparable in both species. This method proved to be less time consuming and this is the need of the researchers. This can be used to carry out the pharmacokinetic analysis of orbifloxacin in the future.
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