This study was performed to evaluate and compare the pharmacokinetic parameters between
two dosage formulations of hesperidin and naringenin: mixture and tablet. Our objective
was to determine that the flavonoid tablet does not significantly modify the pharmacokinetic parameters
compared with the mixture. For this study, we administered 161 mg/kg of either mixture
(Mix-160) or tablet composed of hesperidin and by intragastric administration. Blood microsamples
were collected from tail vein up to 24 h. Serum flavonoid extraction was performed by solid phase
extraction and analyzed by LC-MS/MS of triple quadrupole (QqQ). Serum concentration vs. time
plot showed that data fitted for a first-order model. The pharmacokinetic parameters were calculated
by a noncompartmental model. The results showed that the absorption constant is higher than the
elimination constant. The first concentration was found at five minutes, and minimal concentration
at 24 h after administration, suggesting a enterohepatic recirculation phenomena and regulation of
liver cytochromes’ activity. We did not find meaningful differences between the pharmacokinetic
parameters of both samples. We concluded that tablet form did not interfere with the bioavailability
of hesperidin and naringenin, and it could be a suitable candidate for developing a drug product.
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