Long acting moxifloxacin is a fluoroquinolone drug, exclusively developed for the veterinary medicine. But since the discovery of the drug, there are very few research is available on the pharmacokinetics of long acting moxifloxacin and there is lack of data availability of on retention time. Therefore, we have conducted the study to elucidate retention time of long acting moxifloxacin in healthy patanwadi sheep. The study was conducted on 6 healthy sheep. Blood samples (5 mL) were collected by using heparinized tubes from jugular vein without drug administration. Different standards of long acting moxifloxacin were prepared by using pure form drug. Long acting moxifloxacin standard concentrations in plasma were determined by high performance liquid chromatography with fluorescence detector. In sheep, the retention time was 6.771 min, 6.768 min, 6.765 min, 6.769 min, 6.760 min, 6.760 min, 6.761 min, 6.763 min and 6.761 min at different long acting moxifloxacin standard concentrations 0.025, 0.050, 0.100, 0.500, 1.250, 2.500, 5.000, 10.000 and 20.000 μg/mL, respectively. The mean retention time of long acting moxifloxacin standards was 6.76 ± 0.0023 min in sheep, respectively. The study results indicate that retention time was comparable in both species. This method proved to be less time consuming and this is the need of the researchers. This can be used to carry out the pharmacokinetic analysis of long acting moxifloxacin in the future.
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