The objective of the present study was to evaluate the antioxidant activity, antitumor effect, and antiaging property of\nproanthocyanidins from Kunlun Chrysanthemum flowers (PKCF) grown in Xinjiang. In vitro antioxidant experiments results\nshowed that the total antioxidant activity and the scavenging capacity of hydroxyl radicals (?OH) and 1,1-diphenyl-2-picrylhydrazyl\n(DPPH?) radicals increased in a concentration-dependent manner and were stronger than those of vitamin C. To investigate the\nantioxidant activity of PKCF in vivo, we used serum, liver, and kidney from mouse for the measurement of superoxide dismutase\n(SOD), malondialdehyde (MDA), and total antioxidant capacity (T-AOC). Results indicated that PKCF had antioxidative effect\nin vivo which significantly improved the activity of SOD and T-AOC and decreased MDA content. To investigate the antitumor\nactivity of PKCF, we used H22 cells, HeLa cells, and Eca-109 cells with Vero cells as control. Inhibition ratio and IC50 values were\nmeasured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; PKCF showed great inhibitory activity\non H22 cells and HeLa cells.We also used fruit flies as a model for analyzing the anti-aging property of PKCF. Results showed that\nPKCF has antiaging effect on Drosophila. Results of the present study demonstrated that PKCF could be a promising agent that may\nfind applications in health care, medicine, and cosmetics.
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