Phytochemicals have been presumed to possess prophylactic and curative properties in several pathologies, such as arsenic- (As-)\ninduced immunosuppression. Our aim was to discover a lymphoprotective extract from Lantana grisebachii Stuck. (Verbenaceae)\n(LG). We assessed its bioactivity and chemical composition using cell-based assays. Fractions produced from a hexane extract\nacutely induced nitrite formation in T-activated cell cultures (P < 0.0001). Water extraction released a fraction lacking nitrite\ninducing activity in both lymphocyte types. Aqueous LG was found to be safe in proliferated and proliferating cells. The\ninfusion-derived extract presented better antioxidant capacity in proportion to phenolic amount in lymphocytes (infusive LG-\n1i at 100 ?g/mL), which protected them against in vitro As-induced lymphotoxicity (P < 0.0001). This infusive LG phytoextract\ncontained 10.23 �± 0.43mg/g of phenolics, with 58.46% being flavonoids. Among the phenolics, the only predominant compound\nwas 0.723mg of chlorogenic acid per gram of dry plant, in addition to 10 unknown minor compounds. A fatty acid profile was\nassessed. It contained one-third of saturated fatty acids, one-third of ?9, followed by ?6 (?24%) and ?3 (?4%), and scarce ?7.\nSumming up, L. grisebachii was a source of bioactive and lymphoprotective compounds, which could counteract As-toxicity. This\nsupports its phytomedical use and research in order to reduce As-related dysfunctions.
Loading....