Number of plant species, crude extracts and phytoconstituents were proven for antimicrobial effects and can be used as a natural antiseptic to kill or inhibit the growth of microorganisms when applied to living tissue or skin and reduces the possibilities of infection, sepsis or putrefaction. The objective of the study was to formulate and evaluate the antimicrobial herbal ointment from the herbal crude extracts. The ethanolic extracts of the C. longa, R. cordifolia and aqueous extract of G. glabra plants were taken in different ratios by factorial designing approach and the antimicrobial tests of the extract combinations were performed. The most effective extract combination was determined by comparing the results of the zone of inhibition obtained from different extract ratios on Bacillus subtillus, Escherichia coli, Pseudomonas aeruginosa and Staphylococcous aureus species. The herbal ointment was prepared by incorporation of selected effective extract ratio into the selected ointment base using hot melting method. Prepared herbal ointment was evaluated for various semisolid dosage form parameters, stability study and antimicrobial effect. Herbal ointment was prepared using alcoholic extracts of C. longa, R. cordifolia and aqueous extract of G. glabra with antimicrobial potential.
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