Tulsi leaves have been found to exhibit a wide variety of activities which are very helpful to mankind. In present study, the volatile oil of different species of tulsi (Ocimuma fricanum, Ocimum basillicum, Ocimum gratissimum and Ocimum citriodorum) was taken by hydraulic distillation of the leaves. The diluted volatile distillate was subjected to tests for larvicidal activity. Larvicidal activity test were performed for volatile oil obtained from four different species of tulsi to evaluate the percentage mortality produced by volatile oil of each species of tulsi in the fourth instar larvae of mosquitoes after 24 hrs of exposure. Ocimuma fricanum species was found most efficacious with outcomes of maximum larvicidal activity among all tested groups.
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