Newcastle Disease (ND) is a highly contagious viral disease\nthat has a tremendous negative impact on the poultry industry worldwide.\nPlant extracts were prepared from five different medicinal plants and\napplied against Newcastle Disease Virus (NDV) to evaluate the antiviral\nreplication in Specific Pathogen-Free (SPF) chicken embryos. Three\ndilutions from each plant extract were prepared and mixed with a fixed\ntiter (10\n4\nEID50/mL) of virulent NDV strain Herts 33. The mix was\ninoculated into nine-day-old SPF chicken embryos that were monitored\nfor five days. Real-time PCR and hemagglutination tests were conducted\nto evaluate the activity of NDV and its viral RNA titer. Some plant\nextracts showed a complete inhibition of NDV evidenced by the absence\nof embryo deaths, the absence of HA titer and viral RNA in the allantoic\nfluid. These plant extracts were from Moringa peregrina (leaves), Acacia\ncyanophylla (leaves), Eucalyptus camaldulensis (fruits) and Pistacia\natlantica (leaves and stems). Other plant extracts showed partial\ninhibition of NDV, such as Ceratonia siliqua (leaves) and Eucalyptus\ncamaldulensis (leaves). This experiment shows the potential of using\nmedicinal plants as antiviral agents.
Loading....