Triticum aestivum is also known as wheat grass which is rich in anti oxidants and flavonoids. Due to high antioxidant content and enzyme super oxide dismutase (SOD) which converts dangerous free radical reactive oxygen species into hydrogen peroxide having extra oxygen molecule to kill cancer cells. The aim of this study was to elucidate the in-vitro cytotoxic activity of Triticum aestivum. The cytotoxic activity of the Triticum aestivum extracts were evaluated with the in-vitro hemolytic assay and brine shrimp lethality (lethality assay). In the hemolytic study, the absorbance of the liberated haemoglobin is checked using UV spectrophotometer and in the lethality assay the % mortality was checked by counting the viable nauplii at different time intervals. The Triticum aestivum extracts has shown significant cytotoxic action in the hemolytic assay as well as in lethality assay. The concentration depended cytotoxic activity was observed. 1000 mcg/ml has shown the highest potency. In-vitro cytotoxic hemolytic study for methanolic extract of Triticum aestivum showed concentration dependent effect on erythrocytes which support cytotoxic action of Triticum aestivum. With respect to the effect of the time of exposure, in the lethargy test the highest percentage of cytotoxicity was detected at 12 h or 24 h of exposure. Triticum aestivum could be attributed to the presence of phytochemical in suitable form. So, it can be the next better, safer and cheaper herbal alternate in management of chronic diseases like cancer.
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