The use of plants as medicines represents by far the biggest human use of the natural world. Plants provide the predominant ingredients of medicines in most medical traditions. Changing forest land to agriculture may, in the short term, slightly enhance the nutritional status of some people, yet leads to a loss of important medicinal plants and can expose them to diseases resulting from ecosystem imbalance. Conservation of such an important medicinal plant is crucial need today. A review highlighting various in vitro protocols developement for selected rare and threatened plant species of wild has been done to highlight current research needs and the significance of ex situ conservation in cases where regeneration through conventional methods is difficult to undertake and species are left with low population in the wild.
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