This work deals with, the antioxidant activity of Methanol extracts of H. shulli belonging to family Acanthaceae commonly known as “Kolsundar” were evaluated by various in-vitro antioxidant assay like DPPH ( 1,1-diphenyl-2-picrylhydrazyl) radical scavenging method, reducing power ability, hydrogen peroxide scavenging assay. Total phenolic contents and tannins was estimated using Folin ciocalteau colorimetric method and tannin precipitation method respectively. The comprise antioxidant activities were compared to Standard antioxidants such as DPPH, Ascorbic acid. The Methanolic extract of HS have effective reducing power, free radical scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, and metal chelating activities. This antioxidant property depends on concentration and increasing with increased amount of sample. In addition, total phenolic compounds in the Methanol extract were determined as Gallic acid equivalents. The results obtained in the present study indicated that the H.Schuli is a potential source of natural antioxidant. Although, the tests presented here showed the usefulness of HS extracts as in vitro antioxidants it still needs to be that this extracts show their activity in emulsions, biological systems and health implications. These results suggested that HS had potent antioxidant activities and could be explored as novel natural antioxidant.
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