Non-viral gene delivery system with many advantages has a great potential for the future of\ngene therapy. One inherent obstacle of such approach is the uptake by endocytosis into\nvesicular compartments. Receptor-mediated gene delivery method holds promise to overcome\nthis obstacle. In this study, we developed a receptor-mediated gene delivery system\nbased on a combination of the Pseudomonas exotoxin A (PE), which has a receptor binding\nand membrane translocation domain, and the hyperthermophilic archaeal histone (HPhA),\nwhich has the DNA binding ability. First, we constructed and expressed the rPE-HPhA\nfusion protein. We then examined the cytotoxicity and the DNA binding ability of rPE-HPhA.\nWe further assessed the efficiency of transfection of the pEGF-C1 plasmid DNA to CHO\ncells by the rPE-HPhA system, in comparison to the cationic liposome method. The results\nshowed that the transfection efficiency of rPE-HPhA was higher than that of cationic liposomes.\nIn addition, the rPE-HPhA gene delivery system is non-specific to DNA sequence,\ntopology or targeted cell type. Thus, the rPE-HPhA system can be used for delivering genes\nof interest into mammalian cells and has great potential to be applied for gene therapy.
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