Theproliferation and apoptosis of pulmonary artery smoothmuscle cells (PASMCs) are considered to be key steps in the progression\nof pulmonary arterial hypertension (PAH). MicroRNAs (e.g., miR-29b) have been identified in various diseases to be critical\nmodulators of cell growth and apoptosis by targetingMcl-1 and CCND2. However, the role of miR-29b in PAH remains unknown.\nSo we try to investigate the effect of miR-29b on Mcl-1 and CCND2 protein in PASMCs, analyze the effect of miR-29b on the\nproliferation of PASMCs, and explore the significance of miR-29b in the proliferation, apoptosis, and gene therapy of PAH. It\nwas observed that gene chip analysis showed miR-29b expression in pulmonary artery tissue. The expression of miR-29b was\nsignificantly reduced in PAHmodel mice.MiR-29b inhibited the proliferation of PASMCs and promoted the apoptosis of PASMCs.\nMechanically, miR-29b could inhibit the expression of Mcl-1 and CCND2 protein and silenced Mcl-1 and CCND2 could abolish\nthe change of proliferation and apoptosis of PASMCs. These results demonstrate that miR-29b suppressed cellular proliferation and\npromoted apoptosis of PASMCs, possibly through the inhibition of Mcl-1 and CCND2. Therefore, miR-29b may serve as a useful\ntherapeutic tool to treat PAH.
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