Human parechovirus type 3 (HPeV3) is an etiologic agent of respiratory diseases, meningitis,\nand sepsis-like illness in both infants and adults. Monoclonal antibodies (mAbs) can be a promising\ndiagnostic tool for antigenic diseases such as virus infection, as they offer a high specificity toward a\nspecific viral antigen. However, to date, there is no specific mAb available for the diagnosis of HPeV3\ninfection. In this study, we developed and characterized mAbs specific for HPeV3 capsid protein VP0.\nWe used cell-free, wheat germ-synthesized viral VP0 protein for immunizing BALB/c mice to generate\nhybridomas. From the resultant hybridoma clones, we selected nine clones producing mAbs reactive\nto the HPeV3-VP0 antigen, based on enzyme-linked immunosorbent assay (ELISA). Epitope mapping\nshowed that these mAbs recognized three distinct domains in HPeV3 VP0. Six mAbs recognized\nHPeV3 specifically and the other three mAbs showed cross-reactivity with other HPeVs. Using the\nHPeV3-specific mAbs, we then developed an ELISA for viral antigen detection that could be reliably\nused for laboratory diagnosis of HPeV3. This ELISA system exhibited no cross-reactivity with other\nrelated viruses. Our newly developed mAbs would, thus, provide a useful set of tools for future\nresearch and ensure HPeV3-specific diagnosis.
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