Human growth hormone (hGH) is a single-chain polypeptide that participates in a wide range of biological functions such\r\nas metabolism of proteins, carbohydrates and lipids as well as in growth, development and immunity. Growth hormone deficiency in\r\nhuman occurs both in children and adults. The routine treatment for this condition is administration of recombinant human growth\r\nhormone (rhGH) made by prokaryotes. Since nonglycosylated human growth hormone is a biologically active protein, prokaryotic\r\nexpression systems are preferred for its production. Materials and Methods: Different strains of E.coli were transformed by plasmid\r\ncontaining human growth hormone gene and cultured in different conditions. After induction by IPTG, recombinant human growth\r\nhormone production was assessed using ELISA, dot blotting and western blotting techniques. Results: High levels of rhGH were\r\nproduced using E.coli prokaryotic protein production system. Conclusion: This simple and cost effective production process could\r\nbe recruited for large scale production of rhGH.
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