The present investigation was designed to check antimicrobial activity of leaves Musa sapientum L. and development of suitable dosage form. Leaves of Musa sapientum were collected, air dried, powdered and extracted with ethanol, 50% ethanol and water by maceration method and subjected for phytochemical analysis. Antimicrobial activity of extracts was carried out by the agar well diffusion method and tube dilution method. The extracts were tested on clinical isolates include aerobic, facultative bacteria namely Stapyhlococcus aureus ATCC 25921, Escherichia coli ATCC 25922, Bacillus subtilis and fungus Aspergillus niger. Ethanolic extracts showed greater antimicrobial activity than aqueous and hydroalcoholic extracts. The Minimum inhibitory concentration (MIC) of ethanolic extract varied from species of microorganisms, it was found to be 1.5 to 2.5 mg/ml. Further ethanolic extract was used for preparation of topical formulations such as gel (2.5%) and ointment (2.5%). TLC and HPTLC pattern of extract, gel and ointment was compared which showed no change in Rf and 3D spectrum. Then both the formulations are evaluated for their antimicrobial activity, physical characteristics and stability study.
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