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Inventi Impact: Pharm Biotech & Microbio

Articles

  • Inventi:pbm/18572/16
    Purification, Characterization of L-Methioninase from Candida tropicalis, and Its Application as an\nAnticancer
    01-Jan-1970 Research 2016 : January - March
    Mohsen Helmy Selim, El-Zahraa Karm Eldin, Moataza Mahmoud Saad, El-Sayed Eliwa Mostafa, Yosrea Hassan Shetia, Amany Ahmed Hassabo Anise

    The aim of the present study is to purify L-methioninase from Candida tropicalis 34.19-fold with 27.98% recovery after ion exchange\nchromatography followed by gel filtration.The purified enzyme revealed a single band on SDS-PAGE gel with a molecular weight of\n46 KDa. Its optimum temperature was 45 to 55 and thermal stability was 55âË?Ë?C for 15 min. The enzyme had optimum pH at 6.5 and\nstability at a pH range of 5.5 to 7.0 for 24 hr.The maximum activity was observed with substrate concentration of 30

    How to Cite this Article
    CC Compliant Citation: Mohsen Helmy Selim, El-Zahraa Karm Eldin, Moataza Mahmoud Saad, El-Sayed Eliwa Mostafa, Yosrea Hassan\nShetia, and Amany Ahmed Hassabo Anise, â??Purification, Characterization of L-Methioninase from Candida tropicalis, and Its Application as\nan Anticancer,â? Biotechnology Research International, vol. 2015, Article ID 173140, 10 pages, 2015. doi:10.1155/2015/173140, http://\ncreativecommons.org/licenses/by/3.0/.
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