Dothideomycetes sp. Css035 was used for the production of chitosanase with specific activity 3.41 U/mg that have\nbeen increased by 2.45 fold by the use of 30-70% ethanol concentration as a precipitating agent. The optimal conditions for\nenzymatic hydrolysis of chitosan was found to be, the addition of chitosanase with enzyme/ substrate ratio 0.05 U/mg to 2 ml of\n2% soluble chitosan in a shaking water bath at 55�°C for 4 h with 87% reducing sugar released. The produced\nchitooligosaccharides was analyzed by thin layer chromatography and HPLC and the thin layer chromatography clearly\nvisualized the specificity of the used chitosanase for the production of chitooligosaccharides. The produced\nchitooligosaccharides had more than 90% fibrinolytic activity.
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