Uropathogenic Escherichia coli is the common pathogen to cause urinary tract infections (UTIs)\nand have become multidrug-resistant (MDR) extended-spectrum -lactamase (ESBL) producers.\nThe differences in the antimicrobial susceptibility, 5 bla genes, 12 virulence genes of 87 clinical\nESBL-producing E. coli isolates and genomic variations and sequence types of 18 recurrent and\nrepeated isolates from 9 patients were investigated. The 87 MDR-ESBL isolates collected mainly\nfrom indwelling urinary catheters (IUCs) and UTIs were highly resistant to fluoroquinolones, with\nover 50% of the isolates being resistant to cefepime and piperacillin/tazobactam and a few being\nresistant to carbapenem. These isolates carried at least two of the five bla genes examined, with\nthe highest prevalence (87.4%) found for blaCTX-M (blaCTX-M3-like and blaCTX-M14-like), followed by blaCMY-\n2 (80.5%) and blaSHV (56.3%). The predominant virulence genes were the fimbriae gene fimH\nand the toxin genes cnf1 and hlyA in blood isolates and the capsule gene kpsMTII in UTI and blood\nisolates. Over 80% of the isolates carried yersiniabactin and aerobactin of siderophores. In 18 isolates, the fluoroquinolone-resistant ST131 isolate of pulsotypes I and II with blaCTX-M-15 was\nclonally disseminated in the hospital. The genomic plasticity of these ST131 occurred mainly\nthrough the conjugative plasmids with differences in replicon types A/C, I1, FIA, FIB and Y, size\nand number. In conclusion, MDR ESBL-producing E. coli isolates differed in virulence genes of\nUPEC and antibiotic resistance associated with the sources. Plasmid acquisition and chromosomal\nvariations increase the spread of fluoroquinolone-resistant UPEC ST131 worldwide.
Loading....