The present study was conducted to evaluate the performance of cefoxitin disc diffusion method and oxacillin broth microdilution\nmethod for detection of methicillin resistant S. aureus (MRSA), taking presence of mecA gene as reference. In addition, inducible\nclindamycin resistance and beta-lactamase production were studied and minimum inhibitory concentration (MIC) of vancomycin\nfor S. aureus isolates was determined. A total of 711 nonrepeated pus/wound swab samples from different anatomic locations were\nincluded in the study.The Staphylococcus aureus was identified on the basis of colony morphology, Gram�s stain, and biochemical\ntests. A total of 110 (15.47%) S. aureus isolates were recovered, of which 39 (35.50%) isolates were identified as MRSA by cefoxitin\ndisc diffusion method. By oxacillin broth microdilution method, 31.82% of the Staphylococcus aureus isolates were found to be\nMRSA. However, mecA gene was present in only 29.1% of the isolates. Further, beta-lactamase production was observed in 71.82%\nof the isolates, while inducible clindamycin resistance was found in 10% of S. aureus isolates. The MIC value of vancomycin for S.\naureus ranged from 0.016
Loading....