Procedure ofmanufacturing K. pinnata water extracts containing cecropin P1 (CecP1) fromthe formerly described transgenic plants\nis established. It included incubation of leaves at +4âË?Ë?C for 7 days, mechanical homogenization of leaves using water as extraction\nsolvent, and heating at +70âË?Ë?C for inactivating plant enzymes. Yield of CecP1 (after heating and sterilizing filtration) was 0.3% of total\nprotein in the extract. The water extract of K. pinnata + CecP1 exhibits favorable effect on healing of wounds infected with S. aureus\n(equal to Cefazolin) and with a combination of S. aureus with P. aeruginosa (better than Cefazolin). Wild-type K. pinnata extract\nexhibited evident microbicide activity against S. aureus with P. aeruginosa but itwas substantially strengthened in K. pinnata +CecP1\nextract. K. pinnata extracts (both wild-type and transgenic) did not exhibit general toxicity and accelerated wound recovery. Due to\nimmunomodulating activity, wild-type K. pinnata extract accelerated granulation of the wound bed and marginal epithelialization\neven better than K. pinnata + CecP1 extract. Immunomodulating and microbicide activity of K. pinnata synergizes with microbicide\nactivity of CecP1 accelerating elimination of bacteria.
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