Post-antibiotic effect (PAE) is the continued suppression of bacterial growth following a\nlimited exposure to an antimicrobial agent. The presence of PAE needs consequential consideration\nin designing antibiotic dosage regimens. To understand the behavior of bacteria, PAE provides\ninformation on how long antibiotics are applied to the bacteria. Conventional methods of measuring\nPAE depend on population detection and have limitations for understanding the individual behavior\nof bacteria. To observe the PAE, we utilized an imaging technique with the use of microscopy. Here,\nwe discuss the microscopic image analysis system we used to study the PAE at a single-colony level.\nThe size and number of colonies of bacteria were measured prior to and following antibiotic removal.\nWe could count a single colony, see the development of the settlement prior to and following exposure\nof antibiotics and track the colony by microscopy according to the incubation time and the image\nprocessed by our own image processing program. The PAE of antibiotics was quantified by comparing\nbacteria size and number based on their exposure time. In our study, we discovered that the longer\nexposure of antibiotics causes the bacteria to be suppressedâ??even after washing the antibiotics from\nthe solution. This finding suggests that microscopic imaging detection provides a new method for\nunderstanding PAE. In addition, the behavior of the cell in response to drugs and chemicals and their\nremoval can be examined with the use of single colony analysis.
Loading....