Some novel hydrazone derivatives 6aââ?¬â??o were synthesized from the key intermediate\n4-Chloro-N-(2-hydrazinocarbonyl-phenyl)-benzamide 5 and characterized using IR, 1H-NMR,\n13C-NMR, mass spectroscopy and elemental analysis. The inhibitory potential against two secretory\nphospholipase A2 (sPLA2), three protease enzymes and eleven bacterial strains were evaluated. The\nresults revealed that all compounds showed preferential inhibition towards hGIIA isoform of sPLA2\nrather than DrG-IB with compounds 6l and 6e being the most active. The tested compounds exhibited\nexcellent antiprotease activity against proteinase K and protease from Bacillus sp. with compound\n6l being the most active against both enzymes. Furthermore, the maximum zones of inhibition\nagainst bacterial growth were exhibited by compounds; 6a, 6m, and 6o against P. aeruginosa; 6a, 6b,\n6d, 6f, 6l, 6m, 6n, and 6o against Serratia; 6k against S. mutans; and compounds 6a, 6d, 6e, 6m, and\n6n against E. feacalis. The docking simulations of hydrazones 6aââ?¬â??o with GIIA sPLA2, proteinase K\nand hydrazones 6aââ?¬â??e with glutamine-fructose-6-phosphate transaminase were performed to obtain\ninformation regarding the mechanism of action.
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