Background: In regenerative therapy, self-clotted platelet concentrates, such as platelet-rich fibrin (PRF), are\ngenerally prepared on-site and are immediately used for treatment. If blood samples or prepared clots can be\npreserved for several days, their clinical applicability will expand. Here, we prepared PRF from stored whole-blood\nsamples and examined their characteristics.\nMethods: Blood samples were collected from non-smoking, healthy male donors (aged 27ââ?¬â??67 years, N = 6), and\nPRF clots were prepared immediately or after storage for 1ââ?¬â??2 days. Fibrin fiber was examined by scanning electron\nmicroscopy. Bioactivity was evaluated by means of a bioassay system involving human periosteal cells, whereas\nPDGF-BB concentrations were determined by an enzyme-linked immunosorbent assay.\nResults: Addition of optimal amounts of a 10% CaCl2 solution restored the coagulative ability of whole-blood\nsamples that contained an anticoagulant (acid citrate dextrose) and were stored for up to 2 days at ambient\ntemperature. In PRF clots prepared from the stored whole-blood samples, the thickness and cross-links of fibrin\nfibers were almost identical to those of freshly prepared PRF clots. PDGF-BB concentrations in the PRF extract were\nsignificantly lower in stored whole-blood samples than in fresh samples; however, both extracts had similar\nstimulatory effects on periosteal-cell proliferation.\nConclusions: Quality of PRF clots prepared from stored whole-blood samples is not reduced significantly and can\nbe ensured for use in regenerative therapy. Therefore, the proposed method enables a more flexible treatment\nschedule and choice of a more suitable platelet concentrate immediately before treatment, not after blood\ncollection.
Loading....