Nanoparticles or microparticles created by physical complexation between two\npolyelectrolytes may have a prospective use as an excipient for oral insulin administration. Natural\npolymers such as tragacanth, alginate, dextran, pullulan, hyaluronic acid, gelatin and chitosan\ncan be potential candidates for this purpose. In this research, insulin particles were prepared\nby the inclusion of insulin into a tragacanth hydrogel. The effect of the pH and concentration\nrelationship involving polyelectrolytes offering individual particle size and zeta potential was\nassessed by zetasizer and scanning electron microscopy (SEM). Insulinââ?¬â??tragacanth interactions\nat varying pH (3.7, 4.3, 4.6, or 6), and concentration (0.1%, 0.5%, or 1% w/w) were evaluated by\ndifferential scanning calorimetry (DSC) and ATR Fourier transform infrared (ATR-FTIR) analysis.\nIndividual and smaller particles, approximately 800 nm, were acquired at pH 4.6 with 0.5% of\ntragacanth. The acid gelation test indicated that insulin could be entrapped in the physical hydrogel of\ntragacanth. DSC thermograms of insulinââ?¬â??tragacanth showed shifts on the same unloaded tragacanth\npeaks and suggested polyelectrolyteââ?¬â??protein interactions at a pH close to 4.3ââ?¬â??4.6. FTIR spectra of\ntragacanthââ?¬â??insulin complexes exhibited amide absorption bands featuring in the protein spectra and\nrevealed the creation of a new chemical substance.
Loading....