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Inventi Impact: Animal Models & Cell Assays

Articles

  • Inventi:pac/17/11
    PHYTIC ACID PROTECTS AGAINST 6-HYDROXYDOPAMINE-INDUCED DOPAMINERGIC NEURON APOPTOSIS IN NORMAL AND IRON EXCESS CONDITIONS IN A CELL CULTURE MODEL
    30-Jun-2011 Research 2011 : July - September
    Qi Xu, Anumantha G Kanthasamy, Manju B Reddy

    Iron may play an important role in Parkinson's disease (PD) since it can induce oxidative stress-dependent neurodegeneration. The objective of this study was to determine whether the iron chelator, phytic acid (IP6) can protect against 6-hydroxydopamine- (6-OHDA-) induced apoptosis in immortalized rat mesencephalic dopaminergic cells under normal and iron-excess conditions. Caspase-3 activity was increased about 6-fold after 6-OHDA treatment (compared to control; ?? < . 0 0 1) and 30?�µmol/L IP6 pretreatment decreased it by 38% (?? < . 0 5). Similarly, a 63% protection (?? < . 0 0 1) against 6-OHDA induced DNA fragmentation was observed with IP6 pretreatment. Under iron-excess condition, a 6-fold increase in caspase-3 activity (?? < . 0 0 1) and a 42% increase in DNA fragmentation (?? < . 0 5) with 6-OHDA treatment were decreased by 41% (?? < . 0 1) and 27% (?? < . 0 5), respectively, with 30?�µmol/L IP6. Together, our data suggest that IP6 protects against 6-OHDA-induced cell apoptosis in both normal and iron-excess conditions, and IP6 may offer neuroprotection in PD.

    How to Cite this Article
    CC Compliant Citation: Qi Xu, Anumantha G. Kanthasamy,\nandManju B. Reddy, Phytic Acid protects against 6-\nHydroxydopamine-Induced Dopaminergic Neuron Apoptosis in\nNormal and Iron Excess Conditions in a Cell Culture Model,\nSAGE-Hindawi Access to Research Parkinson's Disease Volume 2011, Article ID 431068.
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