Sciatic nerve crush injury in rat animal model is one of the most common experimental models used in regenerative research.\nHowever, the availability of transgenic mouse for nerve regeneration studies is constantly increasing and, therefore, the shift from\nratmodel to mousemodel is, in some cases, necessary.Moreover, since most of the human nerve lesions occur in the upper limb, it\nis also advantageous to shift from sciatic nerve to median nerve. In this study we described an experimental model which involves\nlesions of the median nerve in the mouse. Data showed that the finger flexor muscle contraction strength, assessed to evaluate\nthe motor function recovery, and reached values not different from the control already 20 days after injury. The degree of nerve\nregeneration evaluated with stereological methods in light microscopy showed that, 25 days after injury, the number of regenerated\nmyelinated fibers was comparable to the control, but they were smaller with a thinnermyelin thickness. Stereological analysis made\nin electron microscopy confirmed these results, although the total number of fibers quantified was significantly higher compared\nto light microscopy analysis, due to the very small size of some fibers that can be detected only in electron microscopy
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