Present study was planned to evaluate cytotoxicity of methanolic extract of E. hirta against NCIH-522 and Vero cell line. Fourier transform infrared spectroscopy (FTIR) analysis of the methanolic extract of E. hirta was performed. 3-(4, 5 dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay was used to performed cytotoxicity study of methanolic extract of E. hirta. Doxorubicin was considered as standard reference drug. Concentrations 1000-0.05 µg/ml of extract and doxorubicin were used in study. FTIR spectrum of methanolic extract of E. hirta was showed seven peaks/centers, viz., 674.541, 1050.082, 1096.320, 1285.406, 1389.313, 1559.797 and 2939.877 at the wavelength region of 4,000.00–650.00 cm−1. The results of present study revealed that 50% cell growth inhibition (IC50) of methanolic extract of E. hirta and doxorubicin was 466.13 µg/ml and 531.14 µg/ml against NCIH-522 cell line and 1375.22 µg/ml and 2392.71 µg/ml against Vero cell line respectively. Methanolic extract was found to be toxic against NCIH-522 and Vero cell line. It is due to the presence of toxic class of phytocompounds demonstrated in FTIR spectrum. Further, there is need to isolate, identify and confirm these toxic phytocompounds from the extract.
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