Background: Animal models of para-renal cancer can provide useful information for the evaluation of tumor\nresponse to loco-regional therapy experiments in solid tumors. The aim of our study was to establish a rabbit\npara-renal cancer model using locally implanted VX2 tumors.\nMethods: In order to generate a rabbit model of para-renal cancer, we established four hind limb donor rabbits by\nusing frozen VX2 tumor samples. Following inoculation, rabbits were monitored for appetite and signs of pain.\nViable tumors appeared as palpable nodules within 2 weeks of inoculation. Tumor growth was confirmed in all\nrabbits by high-resolution ultrasound analysis and histology. Once tumor growth was established, hind limb tumors\nextraction was used for tumor line propagation and para-renal tumor creation. Twenty-one rabbit models bearing\npara-renal cancer were established by implanting VX2 tumor into the para-renal capsula. Tumors developed into\ndiscreet 2ââ?¬â??3 cm nodules within 1ââ?¬â??3 weeks of implantation. Serial renal ultrasonography follow-up, starting 1 week\nafter tumor implantation, was performed. Two weeks after tumor implantation, rabbits were euthanized and tumors\nand other organs were collected for histopathology.\nResults: Tumor growth after VX2 tumor fragment implantation was confirmed in all rabbits by high-resolution\nultrasound (US) imaging examinations of the para-renal regions and was measured with digital caliper. The\npara-renal injection of VX2 tumor fragments, achieved tumor growth in 100% of cases. All data were confirmed by\nhistological analysis.\nConclusions: We generated for the first time, a model of para-renal cancer by surgical tumor implantation of\nVX2 frozen tumor fragments into rabbitââ?¬â?¢s para-renal region. This method minimizes the development of\nmetastases and the use of non-necrotic tumors and will optimize the evaluation of tumor response to loco-regional\ntherapy experiments.
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