Orange Albedo (OW/OE) and grape seed extracts (GW/GE) were considered for their medical potentials against carcinoma cells due to their bioflavonoid derivatives. Cytotoxic activity of the OA and GSEs were investigated against breast (MCF-7) and colorectal (HCT-116) cancer cell lines using MTT assay. Moreover, cell cycle distribution by flow cytometry and RT-PCR were conducted. Data recorded revealed that the IC50 value of OW/OE were 1.50 mg/ml and 14.57 mg/ml respectively against MCF-7 cells, 0.50 mg/ml and 0.88 mg/ml respectively against HCT-116 cells. While the IC50 values of GW/GE against MCF-7 were 1.36 mg/ml and 0.17 mg/ml respectively, HCT-116 cancer cell line were 0.21 mg/ml and 0.80 mg/ml respectively. Flow-cytometry analysis showed MCF-7 arrest in the G2/M phase and S phase post treatment with OW/OE. Both OW/OE and GW/GE exert their effects in MCF-7 and HCT-116 cell lines via Bax, p53 and Casp3 up-regulation and Bcl-2 down regulation in a dose dependent manner. These observations suggest the hypothesis that OW/OE and GW/GE could be used as supplementary medication to the current one against breast and colorectal cancers due to their promising effects on cellular proliferation inhibition and apoptosis induction.
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