Lycium barbarum polysaccharide (LBP) is themajor function component of Lycium barbarum L. and has been previously reported\nto induce the phenotypic and functionalmaturation of dendritic cells (DCs) aswell as activating T lymphocytes. In the current study,\nthe immunologic cytotoxicity promoting effect of LBP was assessed and the underlying mechanism was explored. The impact of\nLBP on the phenotype, maturation, and immunogenicity of DCs was assessed. The activity of Notch pathway which is involved\nin the regulation of LBP on DCs was detected. Afterwards, the influence of LBP on cytotoxicity of DC-mediated cytotoxicity T\nlymphocytes (CTLs) to CT26-WT colon cancer cells was further assessed. Administration of LBP induced the phenotypic and\nfunctional maturation of DCs. After being subjected to LBP, the expression of Notch and Jagged and Notch targets Hes1 and Hes5\nwas all upregulated. The cytotoxicity of DC-mediated CTLs was strengthened by administration of LBP. Additionally, cytotoxicity\nof DC-mediated CTLs on CT26-WT colon cancer cells also increased with effector-target ratio. In conclusion, LBP could induce\nthe phenotypic and functional maturation of DCs via Notch signaling and promote the cytotoxicity of DC-mediated CTLs, which\ncould be employed as a promising adjuvant for cancer immunotherapy.
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