Background: Nanogold has been investigated in a wide variety of biomedical applications because of the antiinflammatory\r\nproperties. The purpose of this study was to evaluate the effects of TPU (Therapeutic Pulsed\r\nUltrasound) with gold nanoparticles (GNP) on oxidative stress parameters and the expression of pro-inflammatory\r\nmolecules after traumatic muscle injury.\r\nMaterials and methods: Animals were divided in nine groups: sham (uninjured muscle); muscle injury without\r\ntreatment; muscle injury + DMSO; muscle injury + GNP; muscle injury + DMSO + GNP; muscle injury + TPU; muscle\r\ninjury + TPU + DMSO; muscle injury + TPU + GNP; muscle injury + TPU + DMSO + GNP. The ROS production was\r\ndetermined by concentration of superoxide anion, modulation of antioxidant defenses was determined by the\r\nactivity of superoxide dismutase, catalase and glutathione peroxidase enzymes, oxidative damage determined by\r\nformation of thiobarbituric acid-reactive substance and protein carbonyls. The levels of interleukin-1b (IL-1b) and\r\ntumor necrosis factor-a (TNF-a) were measured as inflammatory parameters.\r\nResults: Compared to muscle injury without treatment group, the muscle injury + TPU + DMSO + GNP gel group\r\npromoted a significant decrease in superoxide anion production and lipid peroxidation levels (p < 0.050). It also\r\nshowed a significant decrease in TNF-a and IL-1b levels (p < 0.050) when compared to muscle injury without\r\ntreatment group.\r\nConclusions: Our results suggest that TPU + DMSO + GNP gel presents beneficial effects on the muscular healing\r\nprocess, inducing a reduction in the production of ROS and also the expression of pro-inflammatory molecules.
Loading....