Assessment of back titration is often done by veterinarians in animal houses at biological units. The more reliable method we use for this is Spearman and Karber. The calculations involved in the assessment are complex, confusing, time consuming and less easily reproducible. The aim of this research was to design a spreadsheet program based on the steps involved in Spearman and Karber for assessment of rabies virus back titration. This way the laborious steps involved in the method can be easily calculated in a simple, clear, faster, reproducible, accurate and user-friendly manner.
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