Lung cancer is a common cancer, and expression profiling can provide an accurate indication to advance the medical intervention.\r\nHowever, this requires the availability of stably expressed genes as reference. Recent studies had shown that genes that are stably\r\nexpressed in a tissue may not be stably expressed in other tissues suggesting the need to identify stably expressed genes in each\r\ntissue for use as reference genes. DNA microarray analysis has been used to identify those reference genes with low fluctuation.\r\nFourteen datasets with different lung conditions were employed in our study. Coefficient of variance, followed by NormFinder,\r\nwas used to identify stably expressed genes. Our results showed that classical reference genes such as GAPDH and HPRT1 were\r\nhighly variable; thus, they are unsuitable as reference genes. Signal peptidase complex subunit 1 (SPCS1) and hydroxyacyl-CoA\r\ndehydrogenase beta subunit (HADHB), which are involved in fundamental biochemical processes, demonstrated high expression\r\nstability suggesting their suitability in human lung cell profiling.
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