This study aims to apply deep eutectic solvents (DESs) as safe and ecient extraction\nmedia that could yield maximized skin-related bioactivities from a mixture of long-lived trees.\nGinkgo biloba L., Cinnamomum camphora (L.) J. Presl., and Cryptomeria japonica (L.f.) D. Don, native to\nAsia, were examined as potential resources of cosmeceutical products. Various DESs synthesized\nfrom cosmetics-compatible compounds were used to prepare leaf extracts. A DES containing glycerol\nand xylitol yielded the highest extractability for isoquercetin, and was selected as the optimal solvent.\nIsoquercetin has various bioactivities and was found in the extracts of the leaves of all three trees. Then,\na series of mixtures of the tree leaves were prepared according to a simplex-centroid mixture design,\nand their DES-extracts were tested for skin-related activities, including antioxidant, anti-tyrosinase,\nand anti-elastase activities. The mixture design resulted in two special cubic models and one quadratic\nmodel best fitted for describing the antioxidant and anti-elastase activities, and the anti-tyrosinase\nactivity, respectively. Based on the established models, three dierent optimal formulations of the\nthree kinds of tree leaves were suggested for maximized responses. The present strategy, which is\nbased on the simplex-centroid mixture design with a DES as the extraction solvent, could be applied\nto developing new materials from a mixture of natural resources, suitable for the cosmetics and\nrelated fields.
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