The development of functional cosmetics with skin improvement effects from natural\nsources is necessary. In this study, the antioxidant, antiwrinkling, moisturizing, and whitening effects\nof Gardeniae fructus extract (GF) were investigated in keratinocytes, melanocytes, and fibroblast cells.\nAntioxidant activity was determined by a DPPH free radical scavenging assay. MMP-1, MMP-9,\nHAS1, and filaggrin mRNA levels were measured by RT-PCR in keratinocytes and fibroblast cells.\nMITF and tyrosinase protein levels were evaluated by blotting analysis in melanocytes. DPPH free\nradical activity was investigated to determine whether GF showed dose-dependent inhibitory activity.\nGF induced the upregulation of HAS1 and filaggrin mRNA expression in keratinocytes and fibroblast\ncells. GF led to the downregulation of MMP mRNA levels in keratinocytes and fibroblast cells.\nWestern blotting was performed to confirm the whitening-related protein (MITF and tyrosinase)\nlevels induced by GF in melanocytes, and the inhibitory activity was superior to that of the Alpha-MSH\nused for the comparison test. GF showed marked antioxidant, antiwrinkling, skin moisturizing, and\nwhitening activity in keratinocytes, melanocytes, and fibroblast cells. Through the results of these\nexperiments, the applicability of GF as a natural and functional cosmetic material was verified.
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