This study analyzed the antioxidant activity, cell viability, and human skin primary\nirritation test using the hot-water extracts of the Syzygium samarangense. As a result of the\nrecent warmer climate, tropical plants have flourished on Jeju Island, and S. samarangense is\none of these plants known to have biological activities. In this study, the hot-water extract of\nS. samarangense leaf and branch was analyzed. Antioxidant activity was measured by DPPH\n(2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,20-azino-bis(3-ethyl-benzthiazoline-6-sulfonic acid))\nassays, and the DMPD (dimethyl-4-phenylenediamine) radical scavenging activity, nitrite scavenging\nactivity, ferrous-ion chelating activity, cupric reducing antioxidant capacity, reducing power assay,\nferric reducing antioxidant power, total phenol content, and total flavonoid content were also\nmeasured. In addition, cell viability was measured by MTT assay in human keratinocyte cells\n(HaCaT), and the safety of the extract for use on the skin was evaluated in the human skin primary\nirritation test. The antioxidant activities, except DMPD radical scavenging activity and ferrous-ion\nchelating activity, were stronger in the branch extract than in leaf extract, and the total phenol and\nflavonoid contents were also higher in the branch extract. Slight irritation was observed in the human\nskin primary irritation test. However, it was possible to observe sucient antioxidant capacity at a\nconcentration lower than the concentration used in the irritation test; therefore, if the concentration of\nthe extract is appropriately adjusted, this suggests that it is a possible natural material suitable for use\nin cosmetics.
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