Current Issue : April-June
Volume : 2022
Issue Number : 2
Articles : 5 Articles
The aim of presented research is to develop a simple and quick method of spectrophotometric
detection for the determination of doxorubicin hydrochloride in blood and plasma. Anthracycline
antibiotics are among the most effective antineoplastic agents. However, despite their high efficacy
in the treatment of various types of cancer, their administration is limited primarily because they
exhibit myocardial toxicity. This may be a limiting factor in the dosage of medications; nevertheless,
drugs exhibiting this mechanism of action constitute a very important group of chemotherapeutics.
One of the more widely studied antibiotics from the anthracycline group is doxorubicin. It exhibits
the highest antineoplastic activity from among a number of derivative compounds. Because of the
adverse effects of doxorubicin, especially cardiotoxicity, it is important to maintain control of its
concentration in body fluids. The method in the study consists of extraction doxorubicin from the
plasma or blood and measurements of the absorbance of light in the visible light range in a DOX
solution with respect to a reference sample. The research used blood and plasma samples spiked with
doxorubicin to give concentrations...................
AWRK6 was a synthesized peptide developed based on the natural occurring peptide
dybowskin-2CDYa, which was discovered in frog skin in our previous study. Here, a quantitative
determination method for AWRK6 analysis in rat plasma by using liquid chromatography-tandem
mass spectrometry (LC-MS/MS) was established and validated following U.S. FDA guidelines. A
combination of plasma precipitation and liquid–liquid extraction was applied for the extraction. For
pharmacokinetics study, the rats were administrated with AWRK6 via intraperitoneal and intravenous
injection. The prepared plasma samples were separated on an ODS column and analyzed by tandem
MS using precursor-to-product ion pairs of m/z: 533.4!84.2 for AWRK6 and m/z: 401.9!101.1 for
internal standard Polymyxin B sulfate in multiple reaction monitoring mode. AWRK6 concentrations
in rat plasma peaked at about 1.2 h after intraperitoneal injections at 2.35, 4.7 and 9.4 mg/kg
bodyweight. The terminal half-life was around 2.8 h. The absolute bioavailability of AWRK6 was
50% after 3 doses via injection, and the apparent volume of distribution was 4.884 1.736 L. The
obtained determination method and pharmacokinetics profiles of AWRK6 provides a basis for further
development, and forms a benchmark reference for peptide quantification....
The PI3K/AKT/MTOR signalling pathway plays an important role in the growth and
proliferation of tumour cells. N-((3S,4S)-4-(3,4-Difluorophenyl)piperidin-3-yl)-2-fluoro-4-(1-methyl-
1H-pyrazol-5-yl)benzamide (Hu7691) is a new-generation selective AKT inhibitor developed at
Zhejiang University. In this study, we developed an ultra-performance liquid chromatographytandem
mass spectrometry (UPLC-MS/MS) for the measurement of Hu7691 in dog plasma. Plasma
was precipitated with acetonitrile and then separated on a trifunctionally bonded alkyl column.
Excellent separation efficiency and selectivity were achieved by adjusting the mobile phase ratio,
with a total running time of only 5 min. The linear dynamic range of the calibration curve was
5–1000 ng/mL. The method was fully validated, and all performance metrics met the criteria. The
validated method was used for the pharmacokinetic monitoring and bioavailability assessment of
Hu7691 in dogs. The results showed that the area under the curve and peak plasma concentration
of Hu7691 increased with increasing dose (oral 5, 10, 20 mg/kg, intravenous 10 mg/kg), and oral
bioavailabilities were 86.7%, 50.8%, and 50.5%, respectively, indicating a high bioavailability of
Hu7691 in dogs. This provides a test basis for the clinical application of the compound....
Given the growing interest in ascorbic acid (AA), there is a need for a reliable and reproducible
method to measure AA status in the human body. Serum AA concentrations do not
correlate well with tissue levels, but AA levels in leukocytes do. However, a standard method for
clinical application is lacking. This present study describes a method to measure AA in the peripheral
blood mononuclear cells (PBMCs) with hydrophilic interaction liquid chromatography (HILIC). The
method can also be used in plasma and other leukocyte subsets. The measurements of AA in PBMCs
and plasma were performed with HPLC with HILIC separation and UV detection. The sample
preparation involved the isolation of PBMCs and lysis and precipitation with acetonitrile. European
Medicine Agency guidelines for bioanalytic method validation were followed for the evaluation.
A highly precise execution of the method was found with intra- and inter-assay variations at a
maximum of 7.8%. In 40 healthy donors, a mean intracellular AA concentration of 7.9 microgram/108
cells was found in PBMCs. A correlation between plasma and PBMC AA concentration was not
present (r = 0.22). In conclusion, we developed a convenient, reliable, and reproducible method for
the quantitative determination of AA within PBMCs and plasma from human blood....
A specific and sensitive approach using magnetic nanoparticle solid-phase extraction
combined with gas chromatography-mass spectrometry (GC-MS) was carried out and applied in the
simultaneous determination of 16 kinds of synthetic cathinones in human urine. The functionalized
extraction material (Fe3O4/NH2-MWCNTs) was synthesized and factors affecting the extraction
efficiency were all tested. Under the optimized conditions of magnetic nanoparticle solid-phase
extraction, the determination of synthetic cathinones in human urine was carried out with GCMS.
Good linear relationships of 16 kinds of synthetic cathinones were obtained in the range of
0.005–5.00 g/mL with the correlation coefficients (r) ranging from 0.9901 to 0.9979, the limits of
detection were in the range between 0.005 and 0.01 g/mL, and the limits of quantitation were
between 0.01 and 0.02 g/mL. Furthermore, the average intra-day precisions were below 8.90%, the
average inter-day precisions were less than 9.52%, and the average recoveries were between 87.03%
and 99.13%, respectively. The results show the advantages of the approach in the determination of
trace synthetic cathinones in complex matrixes, such as environmentally friendly, fast detection, high
efficiency and sensitivity. The practical application indicated that this method could provide scientific
basis for the determination of drugs of abuse in forensic laboratories....
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