Current Issue : July - September Volume : 2013 Issue Number : 3 Articles : 4 Articles
A simple bioanalytical method was developed to estimate Carbamazepine from human plasma with due consideration of cost effective sensitive and specific liquid chromatography-tandem mass spectrometry method was developed and validated for quantification of carbamazepine and Carbamazepine D10 using as internal standard(IS) in K2EDTA human plasma. Both the analytes were extracted by protein precipitation technique. The chromatographic separation was performed by isocratic on Inert sustain C18 100*, 4.6 mm, 5 µ with a mobile phase of 0.1% glacial acetic acid solution in water: organic mixture (35:65) (v/v) [organic mixture-methanol:acetonitrile (80:20)] followed by detection using mass spectrometry. The protonated analyte was quantitated in positive ionization by multiple reactions monitoring with a mass spectrometer. The method was validated and the lower limit of quantification for Carbamazepine and Carbamazepine D10 was found to be 50 ngmL−1 and 50 ngmL−1 respectively. The mean recovery for Carbamazepine and Carbamazepine D10 ranged from 89.34 to 98.01% and also this method no significant matrix effect on analytes. The method was validated over the concentration range of 50 ng/mL to 8000 ng/mL for both Carbamazepine and Carbamazepine D10 in human plasma with a accuracy of within run and between run in the range of 80-120%. The within run and between run precision of Carbamazepine and Carbamazepine D10 were also within the range of 20% (for LLOQ level) and 15% ( for other than LLOQ) of % CV....
A simple, rapid, sensitive and specific liquid chromatography-tandem mass spectrometry method was developed and validated for quantification of Ramipril and its metabolite Ramiprilat in K2 EDTA human plasma. Both the drugs were extracted by solid phase extraction. Enalpril was used as the internal standard (IS). The chromatographic separation was performed by binary gradient on HYPURITY C18, 3x50 mm,3µ with a mobile phase of 0.1% formic acid solution in water: organic mixture (10:90) (v/v)[organic mixture-methanol:acetonitrile(90:10)] followed by detection using mass spectrometry. The protonated analyte was quantitated in positive ionization by multiple reaction monitoring with a mass spectrometer. The method was validated and the lower limit of quantification for Ramipril and Ramiprilat was found to be 0.390 ngmL−1and 0.395 ngmL−1 respectively. The mean recovery for Ramipril and Ramiprilat ranged from 95.63 to 85.66% and also this method no significant matrix effect on analytes. The method was validated over the concentration range of 0.300 ng/mL to 40.000 ng/mL for both Ramipril and Ramiprilat in human plasma with a accuracy of within run and between run in the range of 80-120%. The within run and between run precision of Ramipril and Ramiprilat were also within the range of 20% (for LLOQ level) and 15% (for other than LLOQ) of % CV....
Febuxostat is a novel nonpurine selective inhibitor of xanthine oxidase, which is currently being developed for the management of hyperuricemia in patients with gout. A simple method was developed and validated for the spectrofluorimetric determination of Febuxostat in human plasma. The thiazole moiety in Febuxostat acts as fluorophore which possesses a native fluorescence that could be measured. Febuxostat produces fluorescence at 366 nm (λem) after excitation at 310 nm (λex) in methanol. The calibration graphs were linear in the concentration ranges 50–600 ng/mL. The results were statistically validated and checked through recovery studies. The method was validated and the lower limit of quantification for Febuxostat was found to be 50 ng/mL. The mean recovery for Febuxostat ranged from 95.07 to 97.37%. The sensitivity of the proposed method allows the determination of investigated Febuxostat in human plasma. The statistical comparisons of the results with the reference methods show a proper agreement and indicate no significant difference in accuracy and precision....
Gatifloxacin is identified as important antibacterial agent of class Quinolone antibiotic, acting by inhibiting the bacterial enzymes DNA gyrase and topoisomerase IV. Clinical studies show that cationic preparations forms complexes with quinolone antibiotics and decrease their absorption to various extents. The objective of this study was to investigate the effect of calcium on oral absorption of gatifloxacin in rats to establish a preclinical food supplement-drug interaction model. Gatifloxacin (400 mg) and Calcium supplement (containing 250 mg calcium) used for the study. The peak plasma concentration i.e. Cmax after administration of gatifloxacin was 5.1667 µg/ml at 3.0 hr (Tmax). The Cmax and Tmax of gatifloxacin administered along with calcium supplement were 3.4119 µg/ml and 3.0 hr, respectively. The relative bioavailability of the gatifloxacin tablet administered along with calcium supplement was 66.04% in comparison with gatifloxacin tablet which is assumed to 100%. This reduction in gatifloxacin bioavailability suggests that calcium may forms complex with gatifloxacin leads to the decrease in bioavailability. This food supplement-drug interaction rat model may be useful in prediction of potential food-drug interactions in humans, and also can be utilized as a preclinical tool for interaction study....
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