Current Issue : July - September Volume : 2014 Issue Number : 3 Articles : 7 Articles
Hydrolysed collagen consists of small peptides with low molecular weight, produced from native collagen found in bones, skin and connective tissue of animals, such as cattle, pigs and fish. Due to its low molecular weight, hydrolysed collagen is highly digestible, easily absorbed and distributed in the human body. If ingested, collagen peptides have been shown to be absorbed in the gut and delivered to the skin and joints. In-vitro and in-vivo studies have shown this may impact the skin ageing process. A double-blinded, randomised, placebo controlled study including 108 healthy volunteer subjects was performed to assess the efficacy of an anti-ageing food supplement containing high dosage of hydrolysed collagen, called Pure GOLD COLLAGEN®. Volunteers consumed the products daily for 12 weeks. Most subjects agreed Pure GOLD COLLAGEN® improved the condition of their skin, hair, nails and joints. The effect on the skin was investigated on a sub-group of the volunteers and significant improvements in the wrinkles were achieved, primarily in the surface area and length of wrinkles. Daily oral consumption of Pure GOLD COLLAGEN® for up to 12 weeks does lead to a detectable improvement in skin health. The observations noted in this preliminary study indicate that Pure GOLD COLLAGEN® is effective in improving the appearance of wrinkles of multiple depths and lengths....
The objective of this in vivo pilot study was to investigate whether differential\nbiomarker analysis from skin tape strips could be used, not only to evaluate the difference\nbetween treated and untreated skin, but also to evaluate the effect of different product\ntreatments. Ten volunteers were included in the study, applying two different basic\nformulations on their forearms. After four weeks of product application, and also after one\nweek of treatment remission, tape strips were collected from the different treatment sites,\nas well as from untreated skin. The biomarkers investigated were selected to cover\ndifferent aspects of epidermal differentiation and in connection with moisturization and\nbarrier function. Levels of Involucrin were increased in both treatments, compared to\nuntreated skin, whereas the levels of Keratin-6 were decreased for both treatments. In\naddition, a pattern for increased levels of Hornerin and Claudin-1 was also detected. There\nwere no significant differences between the two treatments, only for treatment compared to\nuntreated, but there were tendencies for different effect on some of the biomarkers\ninvestigated, differences that may reach significance with increased sample size. The major\ndifferences between the two treatments in this study were seen after one week of product\nremission, although due to too small sample size these differences were not significant...
Photodynamic therapy (PDT) with methyl-aminolevulinate (MAL) is an\napproved non-invasive treatment option for actinic keratoses (AKs). In vivo reflectance\nconfocal microscopy (RCM) is a non-invasive tool for real-time imaging of epidermis and\nsuperficial dermis in vivo that has been previously reported to facilitate the in vivo\nevaluation of skin lesions, including AKs. The aim of this study was to investigate the use\nof in vivo RCM in evaluating AKs response to MAL-PDT. For this reason a total of\n10 biopsy-proven AKs were treated by MAL-PDT, according to standard PDT protocol for\nAKs. RCM investigation was performed before and after PDT and RCM-guided punch\nbiopsies was taken at 3 months in all patients for histopathologic examination. At 3 months\nfollow-up, complete clinical response was observed by clinical examination in 9 out of\n10 lesions and a partial clinical response in 1 lesion. In vivo RCM detected two residual\nAKs in subclinical form, missed by clinical examination. Histological analysis confirmed\nthese results. In vivo RCM may be a new alternative tool for the non-invasive diagnosis of\nAKs and evaluation of AKs response to non-invasive treatments, as MAL-PDT, improving\nthe ability of dermatologists to diagnose AKs even in subclinical stage....
Many changes related to aging at the cellular level may be due to the physiological\ncondition of mitochondria. One of the most common types of damage of mtDNA is the\nso-called ââ?¬Å?common deletionââ?¬Â referring to a deletion of 4977 base pairs. In the skin cells this\nphenomenon probably is caused by oxidative damage of mtDNA induced by UV. The present\nstudy was aimed at evaluating the effect of the antioxidant L-ergothioneine on UV-induced\ndamage in skin cells. The effect of L-ergothioneine on the reduced glutathione level was\nstudied. The presence of the ââ?¬Å?common deletionââ?¬Â in human fibroblasts irradiated with UVA\nand treated with L-ergothioneine was evaluated by a polymerase chain reaction. We have\ndemonstrated that L-ergothioneine enhanced the level of reduced glutathione and protected\ncells from the induction of a photoaging-associated mtDNA ââ?¬Å?common deletionââ?¬Â. In view of\nour results, L-ergothioneine could be an effective skin care and anti-photoaging ingredient....
Acne vulgaris is a common human skin disease characterized by areas of skin with seborrhea (scaly red skin), comedones (blackheads and whiteheads), papules (pinheads), nodules (large papules), pimples and possibly scarring. Demand of herbal formulations has been continuously growing in the world market. The present work deals with the preparation and evaluation of the herbal anti-acne gel containing hydroalcoholic extract of Garlic (Allium sativum L., Alliaceae). The plant has been reported in the literature having good anti-microbial, anti-oxidant and anti-inflammatory activity. Various formulation batches i.e. F1 to F9 were prepared and evaluated for various parameters like colour, appearance, consistency, washability, pH, spreadability and antimicrobial activity. Optimized formulation was compared with the marketed preparation. Amongst all the formulation studied batch F5 was found optimum for all the parameters. It is a very good attempt to establish the herbal gel containing hydro-alcoholic extract of garlic (Allium sativum L., Alliaceae)....
Irritant and allergic contact dermatitis are undesired side effects in the\ndevelopment of drugs and cosmetics as well as after contact with environmental or\nindustrial chemicals. Over the last decades, a great deal of progress has been made in the\ndevelopment of alternative In vitro test to assess these issues. Driven by the 7th\nAmendment to the European Cosmetic Directive, the EU policy on chemicals\n(the registration, evaluation, authorization and restriction of chemicals (REACH) system),\nthe update of the European legislation on the protection of animals used in research,\nand emerging visions and strategies for predicting toxicity, in vitro methods are likely to\nplay a major role in the near future. On 12 December 2013, the European Union Reference\nLaboratory for Alternatives to Animal Testing (EURL ECVAM, part of the European\nCommission Joint Research Centre) published its Recommendation on the Direct Peptide\nReactivity Assay (DPRA) for skin sensitization, capable of distinguishing sensitizers from\nnon-sensitizers. Other assays (i.e., KeratinoSensââ??¢ assay) will follow shortly. While a\nnumber of methods are at various stages of development and use, currently it is not\npossible to rank chemicals for their sensitizing potency, an issue that is important for a full\nsafety assessment. It is expected that a predictive method to totally replace animal testing\nwill be in the form of a test battery comprising molecular, cell-based, and/or computational\nmethods, the so-called ââ?¬Å?Integrated Approaches to Testing and Assessmentââ?¬Â. This review\naims to discuss the state-of-the-art in the field of in vitro assessment of contact sensitizers....
To evaluate the influence of the skin aging critical level on the adult skin\nepidermal functional state, an improved analytical method based on the skin surface\nenergetic measurement (TVS modeling) was developed. Tenskinmetric measurements\nwere carried out non-invasively in controlled conditions by contact angle method using\nonly a water-drop as reference standard liquid. Adult skin was monitored by TVS Observatory\naccording to a specific and controlled thermal protocol (Camianta protocol) in use at\nthe interconnected ââ?¬Å?Mamma Margherita Terme spaââ?¬Â of Terme Euganee. From June to\nNovember 2013, the surface free energy and the epidermal hydration level of adult skin\nwere evaluated on arrival of 265 male and 569 female adult volunteers (51ââ?¬â??90 years of age)\nand when they departed 2 weeks later. Sensitive measurements were carried out at\n0.1 mN/m. High test compliance was obtained (93.2% of all guests). Very interesting\nresults are obtained. The high sensitivity and discrimination power of tenskinmetry\ncombined with a thermal Camianta protocol demonstrate the possibility to evaluate at baseline\nlevel the surface energetic changes and the skin reactivity which occurs on adult skin....
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