Current Issue : October - December Volume : 2014 Issue Number : 4 Articles : 5 Articles
A sensitive and accurate method for quantitative determination of dopamine was introduced. The proposed method is based on\ninhibitory effect of dopamine on the oxidation of thionine by bromate in acidic media. The change in absorbance was followed\nspectrophotometrically at 601 nm. The dependence of sensitivity on the reaction variables was investigated and optimized to obtain\nthe maximum sensitivity. Under optimum experimental conditions, calibration curve was linear over the range 0.2ââ?¬â??103.3 ?gmL?1\nof dopamine.The relative standard deviations (n = 6) of 0.5, 1.0, 5.0, and 30.0 ?gmL?1 of dopamine were 1.13, 1.02, 0.99, and 0.97%,\nrespectively.The limit of detection was 0.057 ????gmL?1 of dopamine.The effect of diverse species was also investigated.Thedeveloped\nmethod was successfully applied for the determination of dopamine in pharmaceutical and biological samples....
The main objectives of this study were (a) to evaluate the in vitro performance of the rapid disintegration tablets as a way to\nimprove the solid dispersions and (b) to study the in vivo pharmacokinetics of the albendazole modified formulation in dogs. Rapid\ndisintegration of tablets seems to be a key factor for efficiency of solid dispersions with regard to improvement of the albendazole\nbioavailability. The in vivo assays performed on dogs showed a marked increase in drug plasma exposure when albendazole was\ngiven in solid dispersions incorporated into rapid disintegration tablets compared with conventional solid dosage form....
The hepatoprotective and antioxidant activity of Bauhinia hookeri ethanol extract (BHE) against CCl4-induced liver injury was\ninvestigated in mice. BHE was administered (500 and 1000mg/kg/day) along with CCl4 for 6 weeks. The hepatic marker enzymes:\nalanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were determined in the serum.\nTheantioxidant parameters: glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase\n(GR), glutathione transferase (GST), and malondialdehyde (MDA) were estimated in the liver homogenate. BHE treatment\nsignificantly inhibited the CCl4-induced increase in ALT (44 and 64%), AST (36 and 46%), ALP (28 and 42%), and MDA (39 and\n51%) levels at the tested doses, respectively. Moreover, BHE treatment markedly increased the activity of antioxidant parameters\nGSH, GPx, GR, GST, and SOD. Histological observations confirmed the strong hepatoprotective activity. These results suggest\nthat a dietary supplement of BHE could exert a beneficial effect against oxidative stress and various liver diseases by enhancing\nthe antioxidant defense status, reducing lipid peroxidation, and protecting against the pathological changes of the liver. The\nhepatoprotective activity of BHE is mediated, at least in part, by the antioxidant effect of its constituents. The active constituents of\nBHE were identified by HPLC-PDA-ESI/MS/MS....
Cerebral malaria (CM) is a severe complication of and a leading cause of death due to Plasmodium falciparum infection. CM is\nlikely the result of interrelated events, including mechanical obstruction due to parasite sequestration in the microvasculature,\nand upregulation ofTh1 immune responses. In parallel, blood-brain-barrier (BBB) breakdown and damage or death of microglia,\nastrocytes, and neurons occurs.We found that a novel formulation of a liposome-encapsulated glucocorticosteroid, ????-methasone\nhemisuccinate (nSSL-BMS), prevents experimental cerebralmalaria (ECM) in amurine model and creates a survival time-window,\nenabling administration of an antiplasmodial drug before severe anemia develops. nSSL-BMS treatment leads to lower levels of\ncerebral inflammation, expressed by altered levels of corresponding cytokines and chemokines. The results indicate the role of\nintegrated immune responses inECMinduction and showthat the newsteroidal nanodrug nSSL-BMS reverses the balance between\ntheTh1 and Th2 responses in malaria-infected mice so that the proinflammatory processes leading to ECM are prevented. Overall,\nbecause of the immunopathological nature of CM, combined immunomodulator/antiplasmodial treatment should be considered\nfor prevention/treatment of human CM and long-term cognitive damage....
Osteopontin (OPN) is involved in the regulation of vascular calcification processes. The aim of this study was to evaluate the\nrelationship between fasting serum OPN concentration and carotid-femoral pulse wave velocity (cfPWV) in geriatric persons.\nFasting blood samples were obtained from93 geriatric persons. cfPWVwere performed by SphygmoCor system. SerumOPN levels\nwere measured using a commercially available enzyme-linked immunosorbent assay. Geriatric adults who had diabetes (P = 0.007)\nor dyslipidemia (P = 0.029) had higher cfPWVlevels than those without diabetes or dyslipidemia.Theunivariable linear regression\nanalysis showed that age (P = 0.002), waist circumference (P = 0.048), body mass index (P = 0.004), systolic blood pressure\n(P= 0.001), diastolic blood pressure (P = 0.036), pulse pressure (P = 0.017), creatinine (P = 0.002), and log-OPN level (P = 0.001)\nwere positively correlated with cfPWV levels, while the high-density lipoprotein cholesterol (HDL-cholesterol) level (P = 0.007)\nand glomerular filtration rate (P = 0.001) were negatively correlated with cfPWV levels among the geriatric adults. Multivariable\nforward stepwise linear regression analysis of the significant variables also showed that log-OPN (? = 0.233, R2 = 0.123, regression\ncoefficient: 1.868, P = 0.011) was still an independent predictor of cfPWV levels in geriatric persons....
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