Current Issue : October - December Volume : 2015 Issue Number : 4 Articles : 9 Articles
In the present study a simple precise, rapid and reproducible Reverse phase high performance liquid chromatography (RP-HPLC) method has been developed and validated. The chromatographic separation was carried out by using reverse phase stainless steel column of 4.6×250 mm Hypersil C18 HPLC column with 5 µm (particles packing) held in thermostat at 25°C. The mobile phase consisted of acetonitrile: Phosphate buffer (0.025M) (75/25 v/v), with a flow rate of 0.8 ml/ min and UV detection at 233 nm and injection volume 20 µl was maintained. The retention time was found to be 3.14 min. The different analytical performance parameters such as linearity, accuracy, precision and sensitivity (limit of detection and limit of quantitation) were determined according to international conference on harmonization ICH Q2 (R1) guidelines. The linearity was observed in the range of 10-100 µg/ml with correlation coefficient, r = 0.9981. The proposed method is rapid, cost-effective and can be used as a quality-control tool for routine quantitative analysis of Metformin HCl....
Aim of the present study was to develop chromatographic method of misoprostol epimer. The developed method was found to be simple, precise, accurate and reproducible. The linearity was in range of 500-3000 ng/spot, with correlation coefficient 0.998. Mean percentage recoveries were in the 99.54 - 100.01 range. Based on the best resolutions, we have selected the methanol: toluene (80:20 v/v) along with drop of acetic acid as a mobile phase. The rf value of epimer was in 0.47±0.02 range at wavelength 286 nm. The validation of proposed method was done as per ICH guidelines and was successfully applied to the estimation of Misoprostol. The proposed chromatographic method was simple and economic....
A selective, accurate, precise and reproducible high performance thin-layer chromatographic (HPTLC) method has been developed and validated for the analysis of glycopyrrolate in tablet dosage form (glycopyrrolate tablet USP). The separation was performed on pre-coated silica gel 60 F254 (Merck) plate using ethyl acetate: water: glacial acetic acid (7:2.0:1.0 v/v/v) as mobile phase. Densitometric analysis was performed in reflectance-absorbance mode at 222 nm. Rf value of the glycopyrrolate was found to be 0.34. The linear regression analysis data for the calibration plot showed good linear relationship with correlation coefficient 0.99 (1-6 µg/band). The limit of detection and quantitation were found to be 0.220 µg/ml and 0.667 µg/ml respectively. The percentage recovery of glycopyrrolate was found to be 99.87%. The statistical parameters and recovery study were carried out and reported....
A simple, rapid, precise, accurate and reproducible high performance liquid chromatographic method was developed for estimation of flurbiprofen and chloramphenicol in eye drops. Chromatographic separation of the drugs was achieved on a C-18 phenomenex column using a mobile phase, methanol and 0.5% K2HPO4 (pH -8, adjusted with ortho phosphoric acid) in the ratio of 70:30 v/v, at a flow rate of 1.1 ml/min. The detection was carried out at 245nm. Chloramphenicol and Flurbiprofen were eluted at the retention time of 4.1 min and 6.6 min. The calibration curve of chloramphenicol and flurbiprofen were linear over the range of 10 µg – 800 μg/ml and 10 µg – 600 μg/ml respectively. The UV spectrophotometric (dual wavelength) method was performed at 213 nm and 262 nm for the determination of Flurbiprofen, 300 nm for the determination of chloramphenicol. The calibration curve of chloramphenicol and flurbiprofen were linear over the range of 10 µg – 800 μg/ml and 10 µg – 300 μg/ml respectively. Both methods were validated according to ICH guidelines. These methods can be applied for determination of titled drugs in its formulation with good accuracy and precision....
The objective of the current study was to develop a simple, accurate, precise and rapid RP-HPLC method and subsequently validate as per ICH guidelines for the determination of abacavir hydrochloride using mobile phase (mixture of Phosphate buffer pH 4.2 and acetonitrile in the ratio of 50:50) as the solvent. The proposed method involves the measurement of retention time at selected analytical wavelength. 218.0 nm was selected as the analytical wavelength. The retention time of abacavir HCl was found to be 2.824. The linearity of the proposed method was investigated in the range of 10-70 μg/ml (r2 = 0.998). The method was statistically validated for its linearity, accuracy and precision. Both inter-day and intra-day variation was found to be showing less than 2% RSD (relative standard deviation) value indicating high grade of precision of the method....
The present manuscript describes simple, accurate, precise and economical dual wavelength and derivative spectrophotometric method for estimation of minoxidil and aminexil in their combined topical solution. Minoxidil and aminexil in combination is used as hair growth stimulant in medical condition of androgenic alopecia. The dual wavelength method describes determination of minoxidil by the absorbance difference between two wavelengths 286.98 nm and 271.66 nm and aminexil by the absorbance difference between 301.35 nm and 290.33 nm. The first order derivative method describes the determination of minoxidil at ZCP of aminexil 296.10 nm and of aminexil at ZCP of minoxidil 280 nm. The linearity was observed in the concentration range of 4-20 μg/ml and 3-15 μg/ml for minoxidil and aminexil respectively for both the methods. These methods were successfully applied for assay of marketed formulation. The suitability of these methods for the quantitative determination of minoxidil and aminexil was proved by recovery study and other validation parameters. The proposed methods were found to be simple and sensitive for the routine quality control analysis of minoxidil and aminexil in their combined topical solutions....
In the present study a simple precise, rapid and reproducible reverse phase high performance liquid chromatography (RP-HPLC) method has been developed and validated and the degradation behaviour of albendazole under different stress conditions were performed. The chromatographic separation was carried out by using reverse phase stainless steel column of 4.6×250 mm hypersil C18 HPLC column with 5 µm (particles packing) held in thermostat at 40°C. The mobile phase consisted of acetonitrile/water (80/20 v/v), with a flow rate of 0.8 ml/ min and UV detection at 294 nm and injection volume 20 ml was maintained. The retention time was found to be 5.472 min. The different analytical performance parameters such as linearity, accuracy, precision and sensitivity (limit of detection and limit of quantitation) were determined according to international conference on harmonization ICH Q2 (R1) guidelines. The linearity was observed in the range of 10-100 µg/ml with correlation coefficient, r = 0.9935. Albendazole active substance was subjected to forced degradation to demonstrate the stability indicating power of the HPLC method. Acidic, basic oxidative degradation were used to assess the stability of the method. The drug was found to degrade in all studied conditions but the extent of degradation was different. The proposed method is rapid, cost-effective and can be used as a quality-control tool for routine quantitative analysis of albendazole....
A simple, rapid and precise reverse phase ultra-performance liquid chromatographic (RP-UPLC) method was developed and subsequently validated for simultaneous estimation of thiocolchicoside and lornoxicam in bulk drug and in a tablet dosage form. The method is based on ultra performance liquid chromatography (UPLC) on a reversed phase column, Waters Acquity BEH C18 (100 × 2.1 mm, 1.7 μm). The separation was carried out using a mobile phase containing a buffer and methanol (35:65 v/v), was pumped at a flow rate of 0.2 ml/min, column temperature at 35℃ using UV-detection at 298 nm. Both the drugs were well resolved on the stationary phase and the retention times were around 0.982 minute for Thiocolchicoside and 1.291 minute for lornoxicam. The method was validated and shown to be linear for both the drugs. The correlation coefficients for thiocolchicoside and lornoxicam are 0.998 and 0.997 respectively....
RP-HPLC method was developed for simultaneous estimation of Bacopaside II and Mangiferin using Kromasil RP-18 column with mobile phase used as a combination of Acetonitrile: 10 mM KH2PO4 (40:60 v/v) adjusted to pH 3.0±0.05 with Orthophosphoric acid at 210 nm. RP-HPLC method was developed and validated. The method successfully separated Bacopaside II and mangiferin. The method was found to be linear in range at 10-60 μg/ml of MGN and 20-120 μg/ml of BCP, LOD and LOQ were found to be 0.12 μg/ml and 0.39 μg/ml for MGN and 0.19 μg/ml and 0.57 μg/ml for BCP and the % recovery was found in the range of 98.08-99.18% and 98.05-99.08% and % Assay was found to be 99.25 and 98.40 for MGN and BCP, respectively. The proposed RP-HPLC method was found to be simple, accurate, economical, robust and reproducible....
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