Current Issue : January - March Volume : 2016 Issue Number : 1 Articles : 5 Articles
Blood stasis (BS) is characterized as a disorder of blood circulation. In traditional Korean medicine (TKM), it is viewed as a cause\nfactor of diseases such as multiple sclerosis and stroke. This study investigated differences in the plasma metabolites profiles of\nsubjects displaying BS or non-BS patterns. Thirty-one patients with cerebral infarction diagnosed with BS and an equal number\nof sex- and age-matched non-BS patients were enrolled. Metabolic profiling was performed using UPLC-MS. The ratio of subjects\nwith a rough pulse and purple coloration of the tongue was higher in patients presenting with BS pattern. Through metabolomics\nanalysis, 82 metabolites that differed significantly between the BS and non-BS pattern were identified, and the two groups were\nsignificantly separated using an orthogonal partial least square-discriminant analysis model (...
The objective of the present study was to study the method validation and determination of eprinomectin in sheep plasma and milk following subcutaneous administration at a dose of 0.2 mg.kg-1. The study was conducted using six healthy lactating sheep. Eprinomectin is a novel and potent antiparasitic animal health drug. This paper describes a method for the determination of eprinomectin in sheep plasma and sheep milk by high-performance liquid chromatography (HPLC) with fluorescence detection. Extraction procedure involved treatment of samples with acetonitrile, than supernatant was cleaned up Suplco SPE cartridge and finally dried elute was derivatized with N-methylimidazole, trifluoroacetic anhydride and acetic acid. Extracted samples were analyzed by HPLC with fluorescence detection of the excitation and emission wavelengths of 355 and 465 nm, respectively. After single dose subcutaneous administration eprinomectin, Inter assay and intra assay mean accuracy of the method were 95.56% and 93.79% for plasma and 89.14% and 87.37% for milk sample, respectively. Where, inter assay and intra assay of precision C. V. was ranging from 0.25% to 3.14% and 1.27% to 8.01% for plasma and 1.11% to 5.46% and 3.55% to 6.46% for sheep milk. The method was used to study the residues of eprinomectin in sheep plasma and milk after subcutaneous administration at a dose of 0.2 mg.kg-1....
Most orally administered polyphenols are metabolized, with very little absorbed\nas aglycones and/or unchanged forms. Metabolic and pharmacokinetic studies are therefore\nnecessary to understand the pharmacological mechanisms of polyphenols. Jumihaidokuto\n(JHT), a traditional Japanese medicine, has been used for treatment of skin diseases including\ninflammatory acne. Because JHT contains various types of bioactive polyphenols, our aim was\nto clarify the metabolism and pharmacokinetics of the polyphenols in JHT and identify active\nmetabolites contributing to its anti dermatitis effects. Orally administered JHT inhibited the\nincrease in ear thickness in rats induced by intradermal injection of Propionibacterium acnes.\nQuantification by LC-MS/MS indicated that JHT contains various types of flavonoids and is\nalso rich in hydrolysable tannins, such as 1,2,3,4,6-penta-O-galloyl glucose. Pharmacokinetic\nand antioxidant analyses showed that some flavonoid conjugates, such as genistein 7-O-glucuronide and liquiritigenin 7-O-glucuronide, appeared in rat plasma and had an\nactivity to inhibit hydrogen peroxide-dependent oxidation. Furthermore, 4-O-methylgallic\nacid, a metabolite of Gallic acid, appeared in rat plasma and inhibited the nitric oxide\nreaction. JHT has numerous polyphenols; it inhibited dermatitis probably via the antioxidant\neffect of its metabolites. Our study is beneficial for understanding in vivo actions of orally\nadministered polyphenol drugs....
A rapid, specific and precise reversed phase high performance liquid chromatographic method, with UV detection, was developed for the quantification of sumatriptan succinate loaded in chitosan solid lipid nanoparticles in rat plasma and brain homogenate samples. Rizatriptan benzoate was used as the internal standard and an efficient liquid-liquid extraction technique was employed for extraction from the plasma and brain homogenates. The drug was analyzed by reversed phase C18 column, using 0.05 M potassium dihydrogen orthophosphate solution (containing 10% v/v acetonitrile, 0.1% v/v triethylamine and pH adjusted to 3.8 with o-phosphoric acid) and acetonitrile in a ratio of 80:20, at a flow rate of 1 ml/min and detection wavelength of 226 nm. The developed method was validated for linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ), robustness and specificity. The method was found to be linear in the concentration range of 1.25-8.75 µg/ml and 2.5-17.5 µg/g for plasma and brain homogenates respectively. The other validation parameters were found to be within the acceptable limit (% RSD less than 2%). LOD and LOQ were obtained as 80 ng/ml and 250 ng/ml respectively. The present developed and validated RP-HPLC method thus offers extensive applicability in bioavailability studies involving sumatriptan succinate in nanoparticles....
Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are increased\nin plasma lipids and blood cell membranes in response to supplementation. Whilst\narachidonic acid (AA) is correspondingly decreased, the effect on other fatty acids\n(FA) is less well described and there may be site-specific differences. In response\nto 12 months EPA + DHA supplementation in doses equivalent to 0ââ?¬â??4 portions of\noily fish/week (1 portion: 3.27 g EPA+DHA) multinomial regression analysis was used to\nidentify important FA changes for plasma phosphatidylcholine (PC), cholesteryl ester (CE)\nand triglyceride (TAG) and for blood mononuclear cells (MNC), red blood cells (RBC) and\nplatelets (PLAT). Dose-dependent increases in EPA + DHA were matched by decreases\nin several n-6 polyunsaturated fatty acids (PUFA) in PC, CE, RBC and PLAT, but were predominantly compensated for by oleic acid in TAG. Changes were observed for all FA\nclasses in MNC. Consequently the n-6:n-3 PUFA ratio was reduced in a dose-dependent\nmanner in all pools after 12 months (37%ââ?¬â??64% of placebo in the four portions group). We\nconclude that the profile of the FA decreased in exchange for the increase in EPA + DHA\nfollowing supplementation differs by FA pool with implications for understanding the impact\nof n-3 PUFA on blood lipid and blood cell biology....
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