Current Issue : January - March Volume : 2016 Issue Number : 1 Articles : 5 Articles
The purpose of this research was to formulate taste masked solid dispersions of mebeverine hydrochloride. A 41.31.21 full factorial design was applied for the preparation of twenty-four drug resonates formulae. Four carriers namely; eudragit E100, eudragit EPO, fructose and sacralose were used (variable X1) each at three drug-carrier ratios (1:5, 1:7 and 1:10), (variable X2). Two solvent evaporation techniques were applied either kneading-oven or rotovap method (variable X3). Solid dispersions were evaluated for taste masking using panel test and UV method, drug loading and in-vitro drug release in salivary and gastric pH. Formula prepared using eudragit E100 at 1:5 drug: carrier ratio by rotovap technique was the formula of choice. It showed the highest desirability factor and highest taste masking with immediate release in gastric pH as well as acceptable drug loading. Drug changed from crystalline to amorphous form as seen by the image analyzer and recorded in powder x-ray diffraction analysis. The characteristic drug peak and bands were significantly changed in DSC and FTIR spectra respectively proving physical and chemical interaction between drug and carriers....
In this study, two types of biodegradable polycation (PAsp(DET) homopolymer\nand PEG-PAsp(DET) copolymer) were applied as vectors for inhalable dry gene powders\nprepared by spray freeze drying (SFD). The prepared dry gene powders had spherical and\nporous structures with a 5~10-m diameter, and the integrity of plasmid DNA could be\nmaintained during powder production. Furthermore, it was clarified that PEG-PAsp(DET)-\nbased dry gene powder could more sufficiently maintain both the physicochemical\nproperties and in vitro gene transfection efficiencies of polyplexes reconstituted after powder\nproduction than PAsp(DET)-based dry gene powder. From an in vitro inhalation study\nusing an Andersen cascade impactor, it was demonstrated that the addition of L-leucine\ncould markedly improve the inhalation performance of dry powders prepared by SFD.\nFollowing pulmonary delivery to mice, both PAsp(DET)- and PEG-PAsp(DET)-based dry\ngene powders could achieve higher gene transfection efficiencies in the lungs compared\nwith a chitosan-based dry gene powder previously reported by us....
The effect of sulphuric acid, phosphoric acid and oxalic acid on pore formation in pharmaceutical aluminum foil by single step anodization technique was observed. The anodization reaction was carried out in each acidic medium by subjecting it to the passage of current for specified time intervals thereby forming a nanoporous film over the anodic surface. The concentration of acid, voltage and the time required for each acidic medium was reflected in the pore formation which was observed by scanning electron microscope. Uniform and highly ordered pore formation over the anodic surface was observed for highly concentrated acidic medium as compared to the weaker acids. The detailed findings and discussions on pore formation by several acidic mediums are discussed in the paper....
Our aim was to optimise the encapsulation of an aqueous bitter melon extract by\nspray-drying with maltodextrin (MD) and gum Arabic (GA). The response surface\nmethodology models accurately predicted the process yield and retentions of bioactive\nconcentrations and activity (R2 > 0.87). The optimal formulation was predicted and validated\nas 35% (w/w) stock solution (MD:GA, 1:1) and a ratio of 1.5:1 g/g of the extract to the stock\nsolution. The spray-dried powder had a high process yield (66.2% �± 9.4%) and high retention\n(>79.5% �± 8.4%) and the quality of the powder was high. Therefore, the bitter melon extract\nwas well encapsulated into a powder using MD/GA and spray-drying....
Background: Heterologous production of natural products in Escherichia coli has emerged as an attractive strategy\nto obtain molecules of interest. Although technically feasible most of them are still constrained to laboratory scale\nproduction. Therefore, it is necessary to develop reasonable scale-up strategies for bioprocesses aiming at the overproduction\nof targeted natural products under industrial scale conditions. To this end, we used the production of the\nantibiotic valinomycin in E. coli as a model system for scalable bioprocess development based on consistent fed-batch\ncultivations.\nResults: In this work, the glucose limited fed-batch strategy based on pure mineral salt medium was used throughout\nall scales for valinomycin production. The optimal glucose feed rate was initially detected by the use of a bio catalytically\ncontrolled glucose release (EnBaseÃ?® technology) in parallel cultivations in 24-well plates with continuous\nmonitoring of pH and dissolved oxygen. These results were confirmed in shake flasks, where the accumulation of\nvalinomycin was highest when the specific growth rate decreased below 0.1 hâË?â??1. This correlation was also observed\nfor high cell density fed-batch cultivations in a lab-scale bioreactor. The bioreactor fermentation produced valinomycin\nwith titers of more than 2 mg LâË?â??1 based on the feeding of a concentrated glucose solution. Valinomycin production\nwas not affected by oscillating conditions (i.e. glucose and oxygen) in a scale-down two-compartment reactor,\nwhich could mimic similar situations in industrial bioreactors, suggesting that the process is very robust and a scaling\nof the process to a larger industrial scale appears a realistic scenario.\nConclusions: Valinomycin production was scaled up from mL volumes to 10 L with consistent use of the fed-batch\ntechnology. This work presents a robust and reliable approach for scalable bioprocess development and represents\nan example for the consistent development of a process for a heterologously expressed natural product towards the\nindustrial scale....
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