Current Issue : October - December Volume : 2016 Issue Number : 4 Articles : 6 Articles
An innovative ratio difference spectrophotometric method was developed and validated for the determination of clonazepam (CLO) and paroxetine hydrochloride (PH) with interfering spectra in combined tablet dosage form without previous separation, showing significant advantages and applicability. By applying the ratio difference method, the difference between the two amplitude values is measured by subtraction the two values at the two selected wavelengths in the ratio spectra, so the noise will be completely cancelled. Determination of CLO was carried out by measuring the amplitude difference at λmax of 295.61 nm and 325.42 nm and PH was measured the by subtracting of amplitude difference at 290.64 nm and 320.97 nm. Clonazepam and paroxetine hydrochloride were determined in the concentration range of 5-30 µg/ml and 10-60 µg/ml respectively. The linear regression analysis for the calibration plots produced r2 = 0.9958 and r2 = 0.9992 for clonazepam and paroxetine hydrochloride respectively. The method was validated according to ICH guidelines and can be applied for routine quality control testing....
A simple reverse phase liquid chromatographic method has been developed and validated for simultaneous determination of losartan potassium and hydrochlorothiazide in bulk and tablet dosage form. The separation was carried out using a mobile phase of acetonitrile: buffer: water taken in 25:65:10 %v/v (adjust pH 2.8 with orthophosphric acid). The column used was reverse phase C18 column (Purospher® STAR Hibar®C18, 4.6 mm x 25 cm, 5 µ particles packing) held at 25°C with flow rate of 1.2 ml min using UV detection at 242 nm. The described method was linear over a concentration range of 30-70 μg/ml and 7.5-17.5 μg/ml for the assay of losartan potassium and hydrochlorothiazide respectively. The retention times of losartan potassium and hydrochlorothiazide were found to be 2.89 and 3.96 min respectively. The limit of quantification (LOQ) for losartan potassium and hydrochlorothiazide were found to be 0.025574 μg/ml and 0.03193 μg/ml respectively and the limit of detection (LOD) values were found to be 0.00844 μg/ml and 0.01054 μg/ml respectively for losaratan potassium and hydrochlorthiazide. This study showed that the proposed RP-HPLC method is simple, rapid, precise, economical and accurate, which is useful for the routine determination of losartan potassium and hydrochlorothiazide bulk drug and tablet dosage form....
Two chemometric methods were developed for the simultaneous determination of metoprolol succinate and hydrochlorothiazide. The chemometric methods applied were principal component regression (PCR) and partial least square (PLS). These approaches were successfully applied to quantify the two drugs in the mixture using the information in the UV absorption spectra of appropriate solutions in the range of 220-275 nm with the interval ∆λ = 1 nm. The model development (calibration) was carried out with set of 25 mixtures and model validation with set of 10 mixtures. The evaluation of calibration model is relied on the coefficient of determination (R2) and root mean square error of calibration (RMSEC). The coefficient of determination (R2) for the relationship between actual values and predicted values of both drugs was higher than 0.995 indicating good accuracy of the developed method while RMSEC values obtained were relatively low which indicate acceptable precision of analytical method. The PCR and PLS methods require neither any separation step, nor any prior graphical treatment of the overlapping spectra of the two drugs in a mixture. The proposed methods are simple, rapid and can be easily used as an alternative analysis tool in the quality control as well as in process control of drugs and formulation....
Chitosan is obtained by alkaline partial deacetylation of chitin. Degree of deacetylation is the most important characteristic influences the physical, chemical and biological properties of chitosan. The FTIR spectroscopy is simple and precise method used to determine the degree of deacetylation of the chitosan sample. Degree of deacetylation was found to be 78.90±0.904 for the chitosan sample and used further for the drug delivery applications....
The method discussed in the present work provides a convenient, precise and accurate way for simultaneous analysis of cilnidipine (CIL) and metoprolol succinate (METO) in its bulk and pharmaceutical dosage form by UV spectrophotometric and RP-HPLC methods. Absorbance maxima of CIL at 240 nm and METO at 223 nm were selected for the analysis. With respect to UV-spectroscopic method, regression analysis shows linearity over the concentration range 1-12 µg/ml for CIL and 3-50 µg/ml for METO with respective correlation co-efficient of 0.999 for both the drugs. The assay for CIL and METO was found to be 90.06±1.7 and 97.57±1.13 respectively. The %RSD value for both CIL and METO was found to be less than 2%. In this study the simultaneous equation of CIL and METO was carried out by simultaneous equation satisfactorily. With respect to RP-HPLC method, HPLC conditions were optimized to obtain an adequate separation of eluted compounds. The system Methanol: Buffer (87:13) with pH 6.9 and 1.0 ml/min flow rate is quite robust. The optimum wavelength for detection was 240 nm and 223 nm at which better detector response for CIL and METO was obtained respectively. The average retention time for CIL was found to be 6.4 min and that for METO is 4.3 min. The calibration was linear in concentration range of 1-10 µg/ml for CIL and that for METO was 5-25 µg/ml. The proposed method was validated in accordance with ICH parameters....
An Iodo derivative of lamivudine was synthesized and a method was developed and validated by UV spectroscopy for its determination. The compound was purified by column chromatography. The derivative was confirmed by chemical tests followed by spectral characterization using UV, FTIR spectral data. The study was focused on the effect of amine group in biological activity. The developed method for its determination was simple and validated as per ICH guidelines. The derivative has shown λmax at 226 nm in UV method. The linearity concentration range for the derivative was found to be 10 - 50 μg/ml and co-relation coefficient was found to be 0.996....
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