Current Issue : January - March Volume : 2018 Issue Number : 1 Articles : 5 Articles
A new naproxen amide prodrug was synthesized and spectrally characterized and a simple, precise, and accurate stabilityindicating\nRP-HPLC method was developed and validated for determination and chemical hydrolysis study of the prodrug. Forced\ndegradation studies were conducted as per the International Conference on Harmonization (ICH) guidelines to establish the\nstability-indicating power of the method. Separations were performed on a C18 column (150 Ã?â?? 4.6mm i.d., 5 ...
A simple, rapid, and selective quantitative nuclear magnetic resonance spectroscopic method was evaluated for the determination\nof the content of fluorinated pharmaceuticals. 19F NMR spectra were either obtained in dimethylsulfoxide-d6 or aqueous\nbuffer, using trifluoroacetic acid as internal standard. Quantification of 13 fluorine-containing pharmaceuticals spanning various\npharmacological classes was accomplished using the proposed method. The method was found to be fit for purpose (interday\nprecision 1.2% relative standard deviation) and may thus be applied for routine analysis and quality control of fluorine-containing\npharmaceuticals due to its simplicity, nondestructive samplemeasurement, reliability, and high specificity. Therefore, 19FNMRmay\nserve as a suitable analytical tool for the identification and selective determination of fluorinated pharmaceuticals used as reference\nmaterials and bulk samples....
The stems of Dendrobium officinale Kimura et Migo (Dendrobii Officinalis Caulis) have a high medicinal value as a traditional\nChinesemedicine (TCM). Because of the limited supply, D. officinale is a high priced TCM, and therefore adulterants are commonly\nfound in the herbal market.Thedried stems of a closely related Dendrobium species, Dendrobium devonianum Paxt., are commonly\nused as the substitute; however, there is no effective method to distinguish the two Dendrobium species. Here, a high performance\nliquid chromatography (HPLC) method was successfully developed and applied to differentiate D. officinale and D. devonianum\nby comparing the chromatograms according to the characteristic peaks. A HPLC coupled with electrospray ionization multistage\nmass spectrometry (HPLC-ESI-MS) method was further applied for structural elucidation of 15 flavonoids, 5 phenolic acids, and 1\nlignan in D. officinale. Among these flavonoids, 4 flavonoid C-glycosides were firstly reported in D. officinale, and violanthin and\nisoviolanthin were identified to be specific for D. officinale compared with D. devonianum.Then, two representative components\nwere used as chemical markers. A rapid and reliable high performance thin layer chromatography (HPTLC) method was applied\nin distinguishing D. officinale from D. devonianum. The results of this work have demonstrated that these developed analytical\nmethods can be used to discriminate D. officinale and D. devonianum effectively and conveniently....
A simple HPLC-UV method has been developed and validated for the quantification of ellagic acid (EA) in ethanol extracts of\nEugenia uniflora L. (Myrtaceae) leaves.Theultrasound-assisted extraction (UAE) optimization was performed using a Box Behnken\ndesign (33) combined with response surface methodology to study the effects of the ethanol concentration (%, w/w), extraction time\n(minutes), and temperature (âË?Ë?C) on the EA concentration. The optimized results showed that the highest extraction yield of EA by\nUAE was 26.0 ...
Thecombination of the powerful antimicrobial agent florfenicol and the nonsteroidal anti-inflammatory flunixin meglumine is used\nfor the treatment of bovine respiratory disease (BRD) and control of BRD-associated pyrexia, in beef and nonlactating dairy cattle.\nThis study describes the development and validation of an HPLC-UV method for the simultaneous determination of florfenicol and\nflunixin, in an injectable preparation with a mixture of excipients. The proposed RP-HPLC method was developed by a reversed\nphase- (RP-) C18e (250mmÃ?â?? 4.6 mm, 5 ...
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