Current Issue : April - June Volume : 2018 Issue Number : 2 Articles : 6 Articles
P. zeylanica is perennial herb that distributed extensively in all over Asia and African region in world. Plumbagin is the major constituent derived from the roots of Plumbago zeylanica L. is a naphthoquinone compound. Plumbagin was isolated from P. zeylanica L. root and identified by HPLC, 1H-NMR, 13C-NMR and Mass. The qualitative and quantitative analysis of plumbagin, a major constituent, chemical and biological marker present in P. zeylanica L. done by using HPLC-UV method. The quality of the marketed and self collected samples of Plumbago zeylanica roots were also quantified by HPLC-UV method. The study suggested that there is a lot of difference in the quality of the drug marketed in different parts of country. HPLC-UV analysis showed the higher concentration of plumbagin in the self collected sample of P. zeylanica (0.51 % w/w) than marketed sample (0.20 %). This paper provides anti microbial activity of isolated plumbagin and plumbagin Beta cyclodextrin complex in colonic environmental microbial flora against microorganism strains i.e. E. coli. and Staphylococcus aureus. Antimicrobial activity of plumbagin and its complex was validated using an in-vitro zone of inhibition model. Plumbagin Beta-cyclodextrin complex exhibited more antimicrobial activity than plumbagin....
The present study was designed to evaluate the antibacterial activities of the petroleum ether, methanol and aqueous\nextracts from the stem, leaf, fruit of Aegle marmelos (Linn.). Extracts were studied using agar well diffusion methods against\nstandard bacterial strains of Salmonella abony, Staphylococcus aureus, Pseudomonas auregenosa and Eschirichia coli. Petroleum\nether extract was found to be more active than methanolic and aqueous extracts against Salmonella abony. Aqueous extract was\nfound more active than the other two against S. aureus, P. auregensa and E. coli. It reveals that the water soluble components of\nthe plant are highly active against the above mentioned microorganism....
Velvet antler (Cervus elaphus) is a typical traditional animal medicine. It is considered to have various pharmacological effects\nincluding stimulation of the immune system, increase in the physical strength, and enhancement of sexual function. This paper\naims to investigate the aqueous extract of velvet antler (AVA) in the mouse models of LPS-induced ALI. Inhibition of NO, TNF-...
Alterations in cardiac energy metabolism play a key role in the pathogenesis of diabetic cardiomyopathy. Hypercholesterolemia\nassociated with bioenergetic impairment and oxidative stress has not been well characterized in the cardiac function under\nglycemic control deficiency conditions. This work aimed to determine the cardioprotective effects of quercetin (QUE) against\nthe damage induced by a high-cholesterol (HC) diet in hyperglycemic rats, addressing intracellular antioxidant mechanisms and\nbioenergetics. Quercetin reduced HC-induced alterations in the lipid profile and glycemia in rats. In addition, QUE attenuated\ncardiac diastolic dysfunction (increased E:A ratio), prevented cardiac cholesterol accumulation, and reduced the increase in\nHC-induced myocyte density. Moreover, QUE reduced HC-induced oxidative stress by preventing the decrease in GSH/GSSG\nratio, Nrf2 nuclear translocation, HO-1 expression, and antioxidant enzymatic activity. Quercetin also counteracted HCinduced\nbioenergetic impairment, preventing a reduction in ATP levels and alterations in PGC-1�±, UCP2, and PPAR�³\nexpression. In conclusion, the mechanisms that support the cardioprotective effect of QUE in rats with HC might be mediated\nby the upregulation of antioxidant mechanisms and improved bioenergetics on the heart. Targeting bioenergetics with QUE can\nbe used as a pharmacological approach to modulate structural and functional changes of the heart under hypercholesterolemic\nand hyperglycemic conditions....
The present study attempts to investigate the cytotoxic activity of ethanol and ethyl acetate extracts of the Moroccan Berberis\nvulgaris and its major component berberine, together with exploring their antioxidant properties. It also consists of studying the\ncombination effect of berberine and S-nitroso-N-acetylpenicillamine (SNAP), a nitric oxide (NO) donor, against the human\nbreast adenocarcinoma cell line (MCF-7). Using the MTT assay, we report a differential cytotoxic effect of ethanol and ethyl\nacetate extracts since the ethanol extract is more cytotoxic than the ethyl acetate one, with IC50 3.54 Ã?¼g/mL and 596.71 Ã?¼g/mL,\nrespectively. Interestingly, no cytotoxic effect was observed against normal cells. Furthermore, these extracts showed a remarkable\nantioxidant activity as measured by the DPPH free radicals scavenging assay. In fact, the IC50 values are 69.65 Ã?¼g/mL and\n77.75 Ã?¼g/mL for the ethanol and ethyl acetate extracts, respectively. In addition, several concentrations of berberine, when\ncombined with the NO donor used at IC30, induced a synergistic cytotoxic activity at concentrations ranging from 8.40 Ã?¼M to\n33.60 Ã?¼M, as revealed by the combination index values, using the Chouââ?¬â??Talalay method. However, at the other concentrations\ntested, an antagonistic effect was observed. The observed cytotoxicity was related to apoptosis induction as demonstrated by the\nannexin-V-streptavidin FITC-staining analysis....
Allergic contact dermatitis (ACD) is a common irritability skin disease, which can be cured by using the Chinese patent medicine.\nTo explore the pharmacological effect of total flavonoids of FructusKochiae (FK) onACD,we used dinitrochlorobenzene- (DNCB-)\ninducedACDrats. Five groupswere used in our experiments.Thenormal group and theDNCB groupwere treatedwith 0.5%CMCNa;\nthe DNCB + hFK group was treated with a high dose of total flavonoids of FK (200mg/kg); the DNCB + lFK group was treated\nwith a low dose of FK (100mg/kg); the DNCB + Pre group was treated with prednisolone acetate (2.5mg/kg). The results showed\nthat FK treatment had significantly attenuated the inflammation induced by DNCB. The increased concentration of cytokines\nincluding IL-6, IL-18, and IFN-...
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