Current Issue : October - December Volume : 2019 Issue Number : 4 Articles : 5 Articles
Allium genus plants, such as leek (Allium porrum), are rich sources of anti-inflammatory\nand anti-oxidant secondary metabolites; this is of interest because it demonstrates their\nsuitability as pharmacological alternatives for inflammatory processes, including allergy treatment.\nThe composition of methanolic leek extract (LE) was analyzed by GC-MS and LC-IT/MS, and\nthe total phenolic content and antioxidant capacity were quantified by colorimetric methods.\nIts pharmacological potential was analyzed in human bronchial epithelial Calu-3 cells, human\nmast cells LAD2, and humanized rat basophiles RBL-2H3. LE exhibited a cytotoxic effect on\nCalu-3 cells and HumRBL-2H3 cells only at high concentrations and in a dose-dependent manner.\nMoreover, LE decreased the degranulation of LAD2 and HumRBL-2H3 cells.... ................
The herbs Plantago asiatica and Clerodendrum trichotomum have been commonly used for\ncenturies in indigenous and folk medicine in tropical and subtropical regions of the world. In this\nstudy, we show that extracts from these herbs have antiviral effects against the respiratory syncytial\nvirus (RSV) in vitro cell cultures and an in vivo mouse model. Treatment of HEp2 cells and A549\ncells with a non-cytotoxic concentration of Plantago asiatica or Clerodendrum trichotomum extract\nsignificantly reduced RSV replication, RSV-induced cell death, RSV gene transcription, RSV protein\nsynthesis, and also blocked syncytia formation. Interestingly, oral inoculation with each herb extract\nsignificantly improved viral clearance in the lungs of BALB/c mice. Based on reported information\nand a high-performance liquid chromatography (HPLC) analysis, the phenolic glycoside acteoside\nwas identified as an active chemical component of both herb extracts. An effective dose of acteoside\nexhibited similar antiviral effects as each herb extract against RSV in vitro and in vivo. Collectively,\nthese results suggest that extracts of Plantago asiatica and Clerodendrum trichotomum could provide a\npotent natural source of an antiviral drug candidate against RSV infection....
Natural bioactive compounds may be used in obese patients because of their ability to\nimpact on various key mechanisms involved in the complex pathophysiological mechanisms of such\ncondition. The aim of this study was to investigate the effect of a Mangifera indica L. leaf extract (MLE)\non adipogenic di_erentiation of murine preadipocyte cells. 3T3-L1 cells were treated during their\ndi_erentiation with various concentrations of (Mangifera indica L.) leaves extract (MLE) (750, 380, 150,\n75 and 35 microg) in order to assess their lipid content, adiponectin production, expression profile of\ngenes involved in lipid metabolism, oxidative stress and inflammation...................
Antibiotics are the elective drugs in bovine mastitis (BM) treatment, despite their low\nrates of efficiency and effectiveness and increasing risk of pathogen resistance. In this sense, it\nis urgent to discover new and effective antimicrobial agents to apply in BM control and even\ntreatment. Plant extracts have been widely recognized as a rich source of phytochemicals with\nantimicrobial potential. Thus, the present work aims to compare the bioactivity of Eucalyptus globulus\nand Juglans regia extracts against Staphylococcus aureus bovine mastitis strains with penicillin G. At\nnon-toxic concentrations, E. globulus exerted a bacteriostatic effect in planktonic cells and J. regia had\nno antimicrobial activity. Penicillin G, at minimum inhibitory concentration (MIC), demonstrated\nbactericidal activity, but just for S. aureus 3, 5, 6 and ATCC 25923, while the other strains seem to have\nacquired resistance. On the other hand, E. globulus and penicillin G in combination demonstrated\nsynergy, being the most effective approach against S. aureus 1, 2 and 4. Thus, penicillin alone\nand in combination with E. globulus or J. regia seems to be promissory strategies to control bovine\nmastitis infections....
Dengue infections are still a worldwide burden, especially in Indonesia. There is no specific\nmedication against the dengue virus. Recently, many types of research have been conducted to\ndiscover a new drug for dengue virus using natural resource extracts. Indonesia, as a tropical country,\nhas a wide biodiversity. There are several medicinal plants in Indonesia that are believed to possess\nanti-dengue activity, such as Myristica fatua, Cymbopogon citratus, and Acorus calamus plants. We\nconducted an in vitro laboratory experiment of several extracts from Indonesian herbs combined\nwith in silico analysis. The extracts were evaluated for safety and antiviral activity in Huh7it-1 cell\nlines, using a single dose of 20 microg/mL and dose-dependent (5, 10, 20, 40, 80 and 160 microg/mL) of plant extracts against dengue virus serotype 2 (DENV-2) NGC strain. The DMSO 0.1% was used as a\nnegative control. The cytotoxic aspect was assessed by counting the cell viability, while the antiviral\nactivity was calculated by counting the average inhibition. The selectivity index (SI) of plant extracts\nwere performed from a ratio of CC50/EC50 value. In silico analysis was conducted to determine\nthe free energy of binding between NS5 of dengue virus with bioactive compounds contained in\nMyristica fatua, Cymbopogon citratus and Acorus calamus extract plants. We determined that all extracts\nwere not toxic against Huh7it-1 cell lines. The methanolic extracts of A. calamus, C. citratus, and M.\nfatua showed inhibition of DENV-2 at a dose of 20 microg/mL to 96.5%, 98.9%, and 122.7%, respectively.\nThe dose-dependent effects showed that M. fatua has the best inhibition activity towards DENV-2.\nMolecular docking result showed that artesunic acid within M. fatua has the best free energy of\nbinding (-7.2 kcal/mol), followed by homoegonol (-7.1 kcal/mol) which was slightly different from\nartesunic acid among others. The methanolic extracts of A. calamus, C. citratus, and M. fatua showed\nprospective anti-dengue activities both in vitro and in silico. Future research should be conducted to\nfind the pure extracts of all useful herbs as a new candidate of antiviral drug....
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