Current Issue : April - June Volume : 2020 Issue Number : 2 Articles : 5 Articles
FDA has recently approved a new fixed-dose combination of amlodipine besylate (AMD) and celecoxib (COX) for the treatment\nof hypertension and osteoarthritis. No analytical method has been reported for analysis of these two analytes so far. Hence, to\nmonitor the quality and quantity in the formulation of AMD and COX a simple, accurate, precise, economical, and eco-friendly\nspectroscopic analytical method has been established. The first method involves the determination of AMD and COX by the first\nderivative UV spectroscopic method with scaling factor 10������.....
The Alpha-defensins (AD) present in synovial fluid have been regarded as constituting the\nmost accurate periprosthetic joint infection (PJI) biomarker. The methods most commonly used for\nestimating AD as a biomarker are the qualitative Synovasure® PJI tests, based on the technique of\nlateral flow, and quantitative enzyme-linked immunosorbent assay (ELISA). Here, we propose a novel\ntest based on detecting Alpha-defensins in synovial fluid by high-performance liquid chromatography\n(HPLC). Synovial fluid was collected from 157 patients diagnosed with PJI, infectious arthritis (IA),\narthrosis, reactive arthritis, and rheumatoid arthritis. AD concentrations in the fluid were determined\nby HPLC, and these same samples were used for additional diagnostic analyses. The results were\nstatistically processed to calculate cutoff concentrations for PJI and IA. HPLC testing showed a\nsensitivity of 94% and a specificity of 92% for diagnosis of PJI, and a sensitivity of 97% and a specificity\nof 87% for diagnosis of IA. Using HPLC, we detected in synovial fluid a combination of three\nAlpha-defensins: human neutrophil peptides HNP1, HNP2, and HNP3. All measured AD concentration\nvalues shown in this work refer to the sum of these three individual concentrations. Our study shows\nthat the HPLC method meets the conditions for measuring precise concentrations of the sum of AD\nand can be recommended as part of a diagnostic array for PJI and IA diagnostics. By this method,\nwe have verified that higher levels of AD in synovial fluid can also be seen in rheumatoid illnesses,\ncrystal arthropathies, and reactive arthritis....
The present work aims at development and validation of reverse phase liquid chromatographic method for determination of ledipasvir and related impurity in bulk and pharmaceutical dosage form. The chromatographic separation was achieved with Zorbax eclipse XDB C18 column (250 mm x 4.6 mm, 5 micro) using buffer (0.03 M potassium dihydrogen phosphate in 1000 ml water) and acetonitrile (40:60). The flow rate of 1.0 ml/min with detection of 264 nm was used in the analysis. The calibration curve of ledipasvir was linear in the range of 120 microgram â?? 360 microgram. The method was validated with respect to linearity, precision, accuracy, specificity, robustness in accordance with ICH guidelines. The method was found to be accurate, specific, precise, simple and robust to analyze ledipasvir and related impurity in bulk and pharmaceutical dosage form....
Herbal preparations from Silybum marianum have been used since the fourth century BC \nin liver disease treatment and against numerous other pathologies. Consumption of silymarin \ncontaining drugs and food supplements continues to increase. Precise, fast, reliable, and complex \ndetermination of all components of silymarin preparations is paramount for assessing its \npharmacological quality. We present here simple and fast HPLC --DAD and LC --MS analytical \nmethods for the determination and quantification of all known silymarin components, including \n2,3 --dehydroflavonolignans that has not been achieved so far. The first method, using a common C18 \ncolumn, allows baseline separation of previously inseparable silychristin A, B, isosilychristin, and \nsilydianin. Moreover, this method allowed detection of three so far unknown silymarin \ncomponents....
The simultaneous determination of betamethasone, dexchlorpheniramine maleate, and sodium benzoate in pharmaceutical syrup\nwas done by using a simple validated HPLC method. The chromatographic separation of the three analytes was done in a C18\ncolumn maintained at 25DegreeC, using a mixture of acetonitrile and 0.02M phosphate buffer solution pH 2.70 (35 : 65, v : v) as mobile phase..................
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